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| Status |
Public on Jan 01, 2022 |
| Title |
GM-CSF cultured and LPS stimulated WT and miR-155 KO DC |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Analyze the transcriptome pattern of miR-155 deficient BMDC in both steady-state and inflammatory conditions, in comparison to WT counterparts.
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| Overall design |
Total bone marrow was cultured in 40 ng/mL GM-CSF enriched RPMI-media for 7 days. A fraction of the floating cells were stimulated with 100 ng/mL LPS for 16 h, after with both unstimulated and stimulate DC were sorted by FACS for DC markers and activation markers (CD86). Unactivated DC from unstimulated samples (CD86-) were isolated together with activated (adherent) DC from LPS stimulated samples (CD86+), and total RNA was isolated.
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| Contributor(s) |
Hedlund S, Yuzawa Y, Fukao T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 10, 2012 |
| Last update date |
Jan 01, 2022 |
| Contact name |
Yoko Fukuda Yuzawa |
| Organization name |
Max-Planck Institute of Immunobiology
|
| Street address |
Stübeweg 51
|
| City |
Freiburg |
| ZIP/Postal code |
79108 |
| Country |
Germany |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA172416 |
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