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Series GSE40120 Query DataSets for GSE40120
Status Public on Aug 15, 2012
Title Microarray analysis of genes activated by mouse CAR and human CAR in vivo.
Organism Mus musculus
Experiment type Expression profiling by array
Summary This dataset is a four-ligand x three-genotype Affymetrix microarray analysis of the regulation of liver genes in the mouse by the constitutive androstane receptor (CAR).
 
Overall design 24 female mice of mixed background (C57BL/6x129Sv) were divided into three groups: wild-type (contains only mouse CAR; mCAR), CAR.KO = knockout mice (mice ablated for mCAR gene; mCAR -/-), and CAR.AH= contains human CAR transgene under the control of the mouse albumin promoter in the mCAR -/- background. Each of the three groups underwent four different treatment regimens: CO = corn oil vehicle control, PB = phenobarbitol (100 mg/kg/day), an anti-convulsant agent which can translocate both mCAR and hCAR into the nucleus to turn on target gene expression, TC = TCPOBOP, a potent non-metabolized ligand of mCAR (3 mg/kg), CITCO = a hCAR specific ligand (30 mg/kg/day). Two mice were used per treatment group and each mouse RNA was used for one chip.
 
Contributor(s) Chua SS, Moore DD
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Submission date Aug 14, 2012
Last update date Jan 08, 2019
Contact name Scott Andrew Ochsner
E-mail(s) sochsner@bcm.edu
Phone 713-798-6227
Organization name Baylor College of Medicine
Department Molecular and Cellular Biology
Lab SPP: Signaling Pathways Project
Street address One Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platforms (1)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
Samples (24)
GSM985983 wild-type_phenobarbitol_rep1
GSM985984 wild-type_phenobarbitol_rep2
GSM985985 CAR.KO_phenobarbitol_rep1
Relations
BioProject PRJNA172862

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40120_RAW.tar 76.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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