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Status |
Public on Aug 15, 2012 |
Title |
Microarray analysis of genes activated by mouse CAR and human CAR in vivo. |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
This dataset is a four-ligand x three-genotype Affymetrix microarray analysis of the regulation of liver genes in the mouse by the constitutive androstane receptor (CAR).
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Overall design |
24 female mice of mixed background (C57BL/6x129Sv) were divided into three groups: wild-type (contains only mouse CAR; mCAR), CAR.KO = knockout mice (mice ablated for mCAR gene; mCAR -/-), and CAR.AH= contains human CAR transgene under the control of the mouse albumin promoter in the mCAR -/- background. Each of the three groups underwent four different treatment regimens: CO = corn oil vehicle control, PB = phenobarbitol (100 mg/kg/day), an anti-convulsant agent which can translocate both mCAR and hCAR into the nucleus to turn on target gene expression, TC = TCPOBOP, a potent non-metabolized ligand of mCAR (3 mg/kg), CITCO = a hCAR specific ligand (30 mg/kg/day). Two mice were used per treatment group and each mouse RNA was used for one chip.
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Contributor(s) |
Chua SS, Moore DD |
Citation missing |
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Submission date |
Aug 14, 2012 |
Last update date |
Jan 08, 2019 |
Contact name |
Scott Andrew Ochsner |
E-mail(s) |
sochsner@bcm.edu
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Phone |
713-798-6227
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Organization name |
Baylor College of Medicine
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Department |
Molecular and Cellular Biology
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Lab |
SPP: Signaling Pathways Project
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Street address |
One Baylor Plaza
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City |
Houston |
State/province |
TX |
ZIP/Postal code |
77030 |
Country |
USA |
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Platforms (1) |
GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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Samples (24)
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Relations |
BioProject |
PRJNA172862 |