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Series GSE40124 Query DataSets for GSE40124
Status Public on Dec 22, 2015
Title Expression data from Amacr knock-out mouse intestine
Organism Mus musculus
Experiment type Expression profiling by array
Summary Bile acids play multiple roles in vertebrate metabolism by facilitating lipid absorption in the intestine and acting as a signaling molecule in lipid and carbohydrate metabolism. Bile acids are also the main route to excrete excess cholesterol out of the body. Alpha-methyl-Coa racemase (Amacr) is one of the enzymes needed to produce bile acids from cholesterol. The mouse model lacking Amacr can produce only minor (less than 10%) amounts of bile acids, but still they are symptomless in normal laboratory conditions.
 
Overall design Cholesterol absorption occurs in the jejunum part of the intestine. In this experiment, the intestines from Amacr-/- and wild-type mice were divided into four equal segments and the endothelial layer was collected by scraping. The RNA from these samples was isolated and the gene expression levels in each segment were compared. 3 biological replicates per genotype/segment combination.
 
Contributor(s) Autio KJ, Kvist AP
Citation(s) 26248199
Submission date Aug 14, 2012
Last update date Dec 22, 2015
Contact name Kaija J Autio
E-mail(s) kaija.autio@oulu.fi
Phone +358 294 48 1142
Organization name University of Oulu
Department Faculty of Biochemistry and Molecular Medicine
Street address P.O.Box 5400
City University of Oulu
ZIP/Postal code 90014
Country Finland
 
Platforms (1)
GPL14661 [Mouse430_2_Mm_EntrezG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Brainarray Version 13]
Samples (24)
GSM986018 wild-type mouse intestine, segment 1, biological rep1
GSM986019 wild-type mouse intestine, segment 1, biological rep2
GSM986020 wild-type mouse intestine, segment 1, biological rep3
This SubSeries is part of SuperSeries:
GSE40125 Expression data from Amacr knock-out mouse liver and intestine
Relations
BioProject PRJNA172866

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40124_RAW.tar 85.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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