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Series GSE40248 Query DataSets for GSE40248
Status Public on Aug 22, 2012
Title Expression data from control and heat shocked mouse spermatocytes and hepatocytes
Organism Mus musculus
Experiment type Expression profiling by array
Summary In somatic cells elevated temperature induces activation of the heat shock transcription factor 1 (HSF1) what leads to heat shock proteins synthesis and cytoprotection. However, in the male germ cells (spermatocytes) upon HSF1 activation, caspase-3 dependent apoptosis is induced and spermatogenic cells are actively eliminated.
To elucidate a mechanism of such diverse HSF1 activity we carried out genome-wide transcriptional analysis in control and heat-shocked cells, either spermatogenic or somatic. As model somatic cells we used hepatocytes that respond to hyperthermia in a classical way by induction of heat shock genes transcription. As spermatogenic cells we used a fraction of cells enriched with spermatocytes, which are the most sensitive to damage in elevated temperatures. Using isolated spermatocytes we avoided the influence of the somatic testicular component on the our final results. Genes that are differently regulated during hyperthermia in both types of cells have been identified.
 
Overall design On Affymetrix gene chip arrays we analyzed RNA isolated from spermatocytes or hepatocytes, either untreated (control) or after heat shock and 2h of recovery at physiological temperature. Analyses were done in triplicate.
 
Contributor(s) Widlak W, Polanska J, Kus-Liskiewicz M, Olbryt M
Citation(s) 23834426
Submission date Aug 21, 2012
Last update date Jul 18, 2013
Contact name Joanna Polanska
E-mail(s) joanna.polanska@polsl.pl
Phone +48322372144
Organization name Silesian University of Technology
Department Automatic Control
Lab Data Mining Group
Street address Akademicka 16
City Gliwice
ZIP/Postal code 44-100
Country Poland
 
Platforms (1)
GPL15967 [Mouse4302_Mm_ENTREZG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Brainarray Version 15.1.0]
Samples (15)
GSM989229 spermatocytes_control_biological rep1
GSM989230 spermatocytes_control_biological rep2
GSM989231 spermatocytes_control_biological rep3
Relations
BioProject PRJNA173340

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Supplementary file Size Download File type/resource
GSE40248_RAW.tar 67.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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