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| Status |
Public on Jun 25, 2013 |
| Title |
Gene expression data of Lsd1fl/fl and Lsd1fl/fl EpoRCre CD71_high / c-Kit_high pro-erythroblasts. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We discovered that mice lacking Lsd1 in the erythroid lineage die in utero of a lethal anemia around embryonic day E13.5. Lsd1 knockout embryos displayed an increase in CD71_high c-Kit_high pro-erythroblasts, followed by a drastic reduction of later maturation stages. To determine the genes altered by Lsd1-loss, CD71_high c-Kit_high pro-erythroblasts from Lsd1fl/fl and Lsd1fl/fl EpoRCre mice were FACS-purified to be analyzed by gene expression profiling.
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| Overall design |
Primary CD71high c-Kithigh pro-erythroblasts were isolated by FACS-sorting from fetal livers of E13.5 Lsd1fl/fl and Lsd1fl/fl EpoRCre embryos. Total RNA from two biological replicates per genotype was extracted and used to hybridize to Affymetrix expression arrays using the Mouse Genome 430A array platform.
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| Contributor(s) |
Kerenyi MA, Orkin SH |
| Citation(s) |
23795291 |
| Submission date |
Aug 22, 2012 |
| Last update date |
May 04, 2018 |
| Contact name |
Marc A. Kerenyi |
| Organization name |
Boston Children's Hospital and Dana Farber Cancer Institute
|
| Department |
Hematology / Oncology
|
| Lab |
Stuart Orkin Laboratory
|
| Street address |
1 Blackfan Street - Karp Research Building
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
|
| Samples (4)
|
| GSM990647 |
Lsd1fl/fl biological replicate #1 [CD71/c-Kit] |
| GSM990648 |
Lsd1fl/fl biological replicate #2 [CD71/c-Kit] |
| GSM990649 |
Lsd1fl/fl EpoRCre biological replicate #1 [CD71/c-Kit] |
| GSM990650 |
Lsd1fl/fl EpoRCre biological replicate #2 [CD71/c-Kit] |
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| This SubSeries is part of SuperSeries: |
| GSE40605 |
Histone Demethylase Lsd1 Represses Hematopoietic Stem and Progenitor Cell Signatures During Blood Cell Maturation. |
|
| Relations |
| BioProject |
PRJNA173407 |
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