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Series GSE4029 Query DataSets for GSE4029
Status Public on May 01, 2006
Title Basonuclin: A novel mammalian maternal effect gene
Organism Mus musculus
Experiment type Expression profiling by array
Summary Basonuclin, which is a zinc-finger protein found in abundance only in the keratinocytes of the stratified epithelium, male germ cells and oocytes, qualifies as a maternal-effect gene because the source of pre-implantation embryonic basonuclin is maternal. Using a transgenic-RNAi approach, we knocked-down basonuclin specifically in mouse oocytes, which led to female sub-fertility. Basonuclin deficiency in oocytes perturbed both RNA polymerase I- and II-mediated transcription and oocyte morphology was affected as evidenced by cytoplasmic and cell surface abnormalities. The affected oocytes, however, could still mature to and arrest at metaphase II and be ovulated, suggesting the impaired pathways were not essential for oocyte development and maturation. Nevertheless, an early embryonic failure in pre-implantation development was identified and likely accounted for the sub-fertility phenotype. These results suggest that basonuclin is a new member of the mammalian maternal-effect genes and interestingly, differs from the previously reported mammalian maternal-effect genes in that it also apparently perturbs oogenesis.
Keywords: RNAi knockdown
Overall design Basonuclin-deficient oocytes were created by a mouse transgenic RNAi approach, which knocks down basonuclin mRNA level specifically in oocytes. Microarray analysis was performed on total RNA from four non-transgenic (control) and six transgenic (basonuclin-deficient) oocyte samples (approximately 50 oocytes per sample).
Contributor(s) Ma J, Zeng F, Schultz RM, Tseng H
Citation(s) 16624857
Submission date Jan 13, 2006
Last update date Feb 11, 2019
Contact name Hung Tseng
Phone 215 898 1981
Fax 215 573 2033
Organization name University of Pennsylvania
Department Dermatology
Lab Hung Tseng
Street address 415 Curie Boulevard Room 242B
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (10)
GSM92221 Non-Transgenic replicate 1
GSM92222 Non-Transgenic replicate 2
GSM92223 Non -Transgenic replicate 7
BioProject PRJNA95135

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