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Series GSE40334 Query DataSets for GSE40334
Status Public on Oct 01, 2012
Title Transcriptome analysis of garlic induced hepatoprotection against alcoholic fatty liver
Organism Mus musculus
Experiment type Expression profiling by array
Summary Alcohol induced fatty liver cause a dangerous health problem and is the major cause of morbidity and mortality worldwide. Garlic (Allium sativum) is documented to possess anti-fatty liver properties. However the exact molecular mechanisms are unknown. The main aim of this experiment is to elucidate the underlying pathways through which garlic ameliorates alcohol induced fatty liver. Dially disulfide and garlic oil were the garlic compounds used in this study. Leiber DeCarli ethanol liquid diet was to induce fatty liver in C57BL/6 mice model. Also the expression impaired by alcohol induced fatty liver is another aim of this study.
Overall design Leiber-Decarli ethanol diet was used to induce fatty liver in male C57BL/6 mice (n=12). For control, Lieber-DeCarli liquid control diet was fed to mice (n=4). The control mice were pair-fed to the ethanol mice. After adaptation, the ethanol fed mice were divided into three groups viz. alcohol (n=4), dially disulfide [DADS] (n=4) and garlic oil [GO] (n=4). The study started with the administration of DADS (15 mg/kg bw) or GO (50 mg/kg bw) mixed in 0.1 ml olive oil through gavage. For the control and alcohol groups, same amount of olive oil (0.1 ml) was gavaged. The mice were gavaged daily for 4 weeks. The mice were euthanized by CO2 and blood was collected by cardiac puncture. Liver, kidney, spleen, lungs and hearts were collected and their weights recorded. A portion of liver was snap frozen in liquid nitrogen (200 mg) for RNA extraction.
Contributor(s) Rajasekaran R, Chen S, Hsiao H, Liu C, Tsai M, Tang X, Kalari KR, Subramanian S, Sheen L
Citation(s) 23066854
Submission date Aug 23, 2012
Last update date Jun 14, 2018
Contact name Lee-Yan Sheen
Phone +886-2-33664129
Fax +886-2-23620849
Organization name National Taiwan University
Department Institute of Food Science and Technology
Lab Lab of Dietary Therapy
Street address No. 1, Sec 4, Roosevelt Road,
ZIP/Postal code 10617
Country Taiwan
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (16)
GSM991393 Diallyl disulfide and alcohol liquid diet, biological replicate 1
GSM991394 Diallyl disulfide and alcohol liquid diet, biological replicate 2
GSM991399 Diallyl disulfide and alcohol liquid diet, biological replicate 3
BioProject PRJNA173777

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Supplementary file Size Download File type/resource
GSE40334_RAW.tar 6.2 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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