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| Status |
Public on Aug 28, 2012 |
| Title |
Expression data from HSCs |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Hepatic stellate cells (HSCs) experience phenotypic transformation, from the quiescent phenotype to the activated one, after different etiologies of liver injury. Liver fibrosis is then occurred upon the activation of HSCs. miR-16 deficiency is identified to be an important characteristic of HSCs activation. We used Affymetrix rat 230 2.0 arrays (Affymetrix, Santa Clara, U.S.A.) to uncover the global alternations of transcriptome under miR-16 restoration.
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| Overall design |
We isolated quiescent hepatic stellate cells (HSCs) from adult male SD rats (normal control group) by in situ perfusion and density-gradient centrifugation. Activated HSCs were separated from rats of fibrosis model group, which were treated by 40% carbon tetrachloride (CCl4) for 8 weeks, by means of liver section, digestion and sequential centrifugation. Quiescent and activated HSCs were then divided into 4 groups at random, namely quiescent HSCs, activated HSCs, pLV-miR-16-treated HSCs and pLV-GFP-treated HSCs. The pLV-miR-16-treated group, pLV-GFP-treated group were infected with pLV-miR-16 and pLV-GFP, respectively.
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| Contributor(s) |
Pan Q, Huang J |
| Citation missing |
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| Submission date |
Aug 27, 2012 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Qin Pan |
| E-mail(s) |
pan_qin@yeah.net
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| Organization name |
Xin Hua hospital
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| Street address |
1665 Kongjiang Rd.
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| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA173817 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40381_RAW.tar |
13.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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