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| Status |
Public on Jul 20, 2013 |
| Title |
siRNA Knockdown of SWI/SNF complex proteins in prostate cancer cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Here we performed transcriptional profiling of the prostate cancer cell lines LNCaP and 22Rv1 comparing non-targeting siRNA treatment versus siRNAs targeting SWI/SNF complex proteins (SMARCA2, SMARCA4, and SMARCB1). Goal was to determine the effect of SWI/SNF knockdown on gene expression in prostate cancer.
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| Overall design |
Two-condition experiment: non-targeting siRNA versus SWI/SNF-siRNA treated cells. Three SWI/SNF proteins were targeted: SMARCA2, SMARCA4, and SMARB1. Biological replicates: 1 control replicate, 2 treatment replicates per SWI/SNF protein. Technical replicates: 1 replicate per SWI/SNF protein. Cell lines: 22Rv1 and LNCaP.
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| Contributor(s) |
Jing X, Iyer MK, Prensner JR |
| Citation(s) |
24076601 |
| Submission date |
Aug 27, 2012 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Matthew Iyer |
| E-mail(s) |
mkiyer@med.umich.edu
|
| Phone |
7346154503
|
| Organization name |
University of Michigan
|
| Department |
Michigan Center for Translational Pathology
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| Lab |
Chinnaiyan Lab
|
| Street address |
5316 CCGC 0940 1400 E. Medical Center Drive
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109-0940 |
| Country |
USA |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE40386 |
SChLAP-1, a long non-coding RNA, antagonizes the SWI/SNF complex and coordinates aggressive prostate cancer. |
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| Relations |
| BioProject |
PRJNA173821 |
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