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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 29, 2012 |
| Title |
Genome-wide maps of Foxa2 binding sites in mouse embryonic stem cell-derived definitive endoderm cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Foxa2 is required for endoderm differentiation into hepatic lineage. The mechanism of activation for Foxa2 during this developmental process has not been elucidated yet. We established an in vitro system to guide ES cells differentiating into definitive endoderm (DE) cells and the following DE cells to early hepatic cells. ChIP-seq assays have been successfully performed to assess Foxa2 binding profile. Over 50% of Foxa2 target genes in DE cells were found being activated in hepatic cells which were at a stage later than DE stage. Therefore, our finding at genome-wide level proved Foxa2 serving as a pioneer factor at DE stage. Furthermore, Foxa2 could specifically induce H3K4me2 modifications to the promoter/enhancer regions of many hepatic genes to pre-mark the chromatin, and determine the hepatic lineage differentiation competence. Our study illustrated the wide existence of Foxa2’s pioneer factor function and uncovered the correlation between pioneer factor and chromatin pre-mark. These findings will be helpful for understanding the developmental process of hepatogenesis and efficiently controlling Foxa2 during hepatic induction for generating functional hepatocytes.
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| Overall design |
Examination of Foxa2 binding sites in mESC-derived DE cells
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| Contributor(s) |
Xu C, Lyu X, Chen EZ, He Z, Li G, Ma Y, Hui L, Xie B, Gao Y, Ding X, Hu Y, Hu P, Han JJ, Wang X |
| Citation(s) |
22750789 |
| Submission date |
Aug 28, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Chenhuan Xu |
| E-mail(s) |
chxu02@gmail.com
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| Organization name |
Beijing Institute of Genomics
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| Street address |
1 Beichen West Road
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA173922 |
| SRA |
SRP015203 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40423_RAW.tar |
560.0 Kb |
(http)(custom) |
TAR (of BED) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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