|
| Status |
Public on Jan 08, 2013 |
| Title |
Gene expression analysis of Ncor1 muscle-specific knockout and PGC-1alpha muscle-specific transgenic skeletal muscle |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
In the present study we have studied the mechanistic and functional aspects of NCoR1 function in mouse skeletal muscle. NCoR1 muscle-specific knockout mice exhibited an increased oxidative metabolism. Global gene expression analysis revealed a high overlap between the effects of NCoR1 deletion and peroxisome proliferator-activated receptor (PPAR) gamma coactivator 1alpha (PGC-1alpha) overexpression on oxidative metabolism in skeletal muscle. The repressive effect of NCoR1 on oxidative phosphorylation gene expression specifically antagonizes PGC-1alpha-mediated coactivation of ERRalpha. We therefore delineated the molecular mechanism by which a transcriptional network controlled by corepressor and coactivator proteins determines the metabolic properties of skeletal muscle, thus representing a potential therapeutic target for metabolic diseases.
|
| |
|
| Overall design |
Gene expression of a total of 20 gastrocnemius samples from control (CON, n = 5), NCoR1 muscle-specific knockout (NCoR1 MKO, n = 5), wild type (WT, n = 5) and PGC-1alpha muscle-specific transgenic (PGC-1alpha mTg, n = 5) adult male mice was analyzed using GeneChip® Gene 1.0 ST Array System (Affymetrix). NCoR1 MKO and PGC-1alpha mTg samples were compared to CON and WT samples, respectively.
|
| |
|
| Contributor(s) |
Handschin C |
| Citation(s) |
23028049 |
| Submission date |
Aug 28, 2012 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Joaquín Pérez-Schindler |
| E-mail(s) |
j.perezschindler@unibas.ch
|
| Organization name |
University of Basel
|
| Department |
Biozentrum
|
| Street address |
Klingelbergstrasse 50 / 70
|
| City |
Basel |
| ZIP/Postal code |
4056 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (20)
|
|
| Relations |
| BioProject |
PRJNA174100 |
Less...
More...