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Series GSE40481 Query DataSets for GSE40481
Status Public on Mar 01, 2014
Title Time-dependent network analysis reveals molecular targets underying the development of diet-induced obesity and non-alcoholic steatohepatitis.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Core diet-induced obesity networks were constructed using Ingenuity pathway analysis (IPA) based on 332 high-fat diet responsive genes identified in liver by time-course microarray analysis (8 time-points over 24 weeks) of high-fat diet fed mice compared to normal diet fed mice. IPA identified five core diet-induced obesity networks with time-dependent gene expression changes in liver. When we merged core diet-induced obesity networks, Tlr2, Cd14 and Ccnd1 emerged as hub genes associated with both liver steatosis and inflammation and were altered in a time-dependent manner. Further protein-protein interaction network analysis revealed Tlr2, Cd14 and Ccnd1 were inter-related through the ErbB/insulin signaling pathway. Dynamic changes occur in molecular networks underlying diet-induced obesity. Tlr2, Cd14 and Ccnd1 appear to be hub genes integrating molecular interactions associated with the development of NASH. Therapeutics targeting hub genes and core diet-induced obesity networks may help ameliorate diet-induced obesity and NASH.
 
Overall design Total RNA obtained from isolated liver of C57BL/6J mice fed normal diet or high fat diet for 0, 2, 4, 6, 8, 12, 16, 20 and 24 weeks.
 
Contributor(s) Shin S, Choi M
Citation(s) 23149694
Submission date Aug 30, 2012
Last update date Jan 16, 2019
Contact name Myung-Sook Choi
E-mail(s) mschoi@knu.ac.kr
Phone 821085881950
Organization name Kyungpook National University
Department Food and Nutrition
Lab Nutrigenomics
Street address 1370, Sankyuk-Dong, Buk-gu
City Daegu
ZIP/Postal code 702-701
Country South Korea
 
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (51)
GSM994787 Baseline (0 week) replicate 1
GSM994788 Baseline (0 week) replicate 2
GSM994789 Baseline (0 week) replicate 3
Relations
BioProject PRJNA174123

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40481_RAW.tar 15.8 Mb (http)(custom) TAR
GSE40481_non-normalized.txt.gz 14.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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