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Series GSE40502 Query DataSets for GSE40502
Status Public on Feb 28, 2014
Title Transcription and enhancer profiling in human monocyte subsets
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Human blood monocytes comprise at least three subpopulations that differ in phenotype and function. Here we generated global maps of H3K4me1 and H3K27ac deposition for classical and nonclassical monocytes defining enhanceosomes of the two major subsets. In combination with HeliScopeCAGE data for all three subpopulations we identify differential regulatory elements (including promoters and putative enhancers) that were associated with subset-specific motif signatures corresponding to different transcription factor activities and exemplarily validate a novel downstream enhancer of the CD14 locus.
 
Overall design ChIP-seq of 2 histone marks in "classical" CD14++CD16- and "nonclassical" CD14dimCD16+ human blood monocytes
 
Contributor(s) Schmidl C, Rehli M
Citation(s) 24671955
Submission date Aug 30, 2012
Last update date May 15, 2019
Contact name Michael Rehli
E-mail(s) michael.rehli@klinik.uni-r.de
Organization name University Hospital Regensburg
Department Internal Med III
Street address F.-J.-Strauss-Allee 11
City Regensburg
ZIP/Postal code 93042
Country Germany
 
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (10)
GSM995353 H3K4me1 CD14+CD16- donor A
GSM995354 H3K4me1 CD14+CD16- donor D
GSM995355 H3K27ac CD14+CD16- donor A
Relations
BioProject PRJNA174187
SRA SRP015328

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40502_RAW.tar 1.7 Gb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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