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Series GSE40503 Query DataSets for GSE40503
Status Public on Jun 20, 2013
Title Study of transcriptional content in exosomes derived from untreated and growth factor-treated cultured cardiomyocytes (HL-1)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Introduction: Exosomes are nano-sized extracellular vesicles, released from various cells, which can stimulate or repress responses in target cells. We have recently shown that cultured cardiomyocytes release exosomes and that they, in turn, are involved in facilitating events in target cells by alteration of gene expression. We investigated whether external stimuli of the cardiomyocyte might influence the released exosome characteristics.

Material and Methods: Exosomes were isolated from media collected from cultured cardiomyocyte (HL-1) cells with or without growth factor treatment (TGF-beta2 and PDGF-BB), with a series of differential centrifugations. The exosomes were characterized with dynamic light scattering (DLS) and Western blot and analysed with Illumina whole genome microarray gene expression.

Results: An average size of 50-80 nm in diameter with no difference between treatment groups was found. Analysis of the mRNA content revealed 623 transcripts in the control group, 691 in the TGF-beta2-treated group and 362 in the PDGF-BB-treated group. 235 transcripts were common for all three groups.

Conclusion: We conclude that there is a difference in mRNA content between exosomes derived from cultured cardiomyocytes stimulated with growth factors. We also conclude that all exosomes contain a basic package consisting of ribosomal transcripts and mRNAs coding for proteins with functions within the energy supply system.
 
Overall design To study if the transcriptional content in exosomes derived from untreated and growth factor-treated cultured cardiomyocytes (HL-1) differ, and if so, can this difference be explained, 4 control (untreated) exosome samples, 4 TFG-beta2-treated cardiomyocyte-derived exosome samples and 4 PDGF-BB-treated cardiomyocyte-derived exosomes were studied.
 
Contributor(s) Gennebäck N, Hellman U, Malm L, Larsson G, Waldenström A, Mörner S
Citation(s) 24009898
Submission date Aug 30, 2012
Last update date Jun 14, 2018
Contact name Nina Gennebäck
E-mail(s) nina.genneback@ncl.ac.uk
Organization name Umeå University
Department Public health and clinical medicin/medicin
Street address NUS 6M
City Umeå
ZIP/Postal code 901 86
Country Sweden
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (12)
GSM995363 Control, rep1
GSM995364 Control, rep2
GSM995365 Control, rep3
Relations
BioProject PRJNA174227

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40503_RAW.tar 3.1 Mb (http)(custom) TAR
GSE40503_non-normalized.txt.gz 3.9 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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