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Series GSE40551 Query DataSets for GSE40551
Status Public on Dec 10, 2012
Title Effects of a 8-week training on human skeletal muscle
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Context: Exercise training is a plausible model for identification of molecular mechanisms that cause metabolic-related changes in human skeletal muscle.

Objective: The goal was to explore the molecular basis of the adaptation of skeletal muscle to exercise training.

Design and Intervention: Obese male subjects were subjected to an individualized supervised training program targeted in order to optimize lipid oxidation during 8 weeks.

Main Outcome Measures: Primary outcome measures were gene expression profiling of skeletal muscle. Body composition, oral glucose tolerance test, Resting metabolic rate, respiratory quotient, maximal oxygen uptake and metabolic biochemistry were also assessed.
Overall design Overall Design
The obese (BMI 30-36) male volunteers (age 32-42) were asked to refrain from vigorous physical activity 48h before presenting to the clinical investigation centre, and ate a weight-maintaining diet consisting of 35% fat, 16% protein, and 49% carbohydrates two days before the experiment. Muscle biopsies of Vastus Lateralis weighing 60–100 mg were obtained using the Bergstrom technique, cleaned and snap-frozen in liquid nitrogen. Resting metabolic rate, respiratory quotient and maximal oxygen uptake were assessed. The subjects were investigated at baseline and after 8 weeks of supervised aerobic exercise training program consisting of daily sessions of 45-60 min of endurance exercise, 5 days a week, at least 48-72h after the last acute exercise bout. Skeletal muscle biopsies were obtained at the beginning and at the end of the protocol. Transcriptome analysis compared 8 subjects before vs. after training using arrays using a common reference design (Cy5 dye was incorporated into all muscle RNA samples, while a reference RNA pool made of the mix of commercial human liver, adipose tissue and skeletal muscle RNA was labelled with Cy3 dye (Applied Biosystems/Ambion, Foster City, USA)( and whole genome 4x44k oligonucleotide arrays (Agilent Technologies).
Contributor(s) Viguerie N, Montastier E, Besse-Patin A, Combes M, Moro C, Langin D
Citation(s) 23114600
Submission date Sep 03, 2012
Last update date Dec 11, 2012
Contact name Nathalie Viguerie
Organization name INSERM
Department I2MC U1048
Lab Obesity Research Laboratory
Street address Avenue Poulhes
City Toulouse
ZIP/Postal code 31400
Country France
Platforms (1)
GPL16022 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Gene Symbol Version)
Samples (16)
GSM994975 Skeletal muscle from obese male 18, control
GSM995422 Skeletal muscle from obese male 18 after 8-week training
GSM995435 Skeletal muscle from obese male 3, control
BioProject PRJNA174449

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40551_RAW.tar 178.5 Mb (http)(custom) TAR (of GPR)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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