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Status |
Public on Sep 05, 2013 |
Title |
Id1 knockdown in primary human glioma stem cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Abnormal activation of stemness factors is a crucial signature of cancer stem cells (CSCs), a highly tumorigenic subpopulation in malignant tumors. However, it is unclear whether multi-signaling pathways are activated in CSCs, as like normal stem cells. I would like to report that an inhibitor of differentiation 1 (ID1) activates intracellular multi-signaling involved in proliferation, genesis, and maintenance of glioma stem cells (GSCs) by suppression of Cullin3, an E3 ubiquitin ligase that degrades Cyclin E and components of SHH and WNT signaling. ID1 inhibits BMP-dependent differentiation of GSCs by activation of BMPR2-targeting miR17/20a. ID1HIGH-Cullin3LOW signature correlates with a poor prognosis of GBM patients with a significant association to gene signatures enriched in EGF, WNT, SHH, and BMP signaling. Combinational inhibition of GSC intracellular multi-signaling network increases tumor-bearing mice survival. These results provide insights on molecular and cellular basis of GSC biology, and also suggest necessity of multi-signaling inhibition for GSCs therapy.
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Overall design |
Two human primary glioma stem cells (GSCs) such as GSC2 and GSC8 were isolated from two individual primary human glioma specimens. The GSCs were directly transfected with pSuper-GFP-ID1-shRNA and pSuper-GFP-Scrambled-shRNA using FuGENE 6 reagent (Roche). The RNA extraction in these cells was used to analyze gene expression.
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Contributor(s) |
Jin X, Jin X |
Citation(s) |
27477274 |
Submission date |
Sep 05, 2012 |
Last update date |
May 14, 2017 |
Contact name |
Xiong Jin |
E-mail(s) |
pierce0701@gmail.com
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Organization name |
Henan University
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Department |
School of Pharmacy
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Lab |
Henan Key Laboratory of Brain Targeted Bio-nanomedicine
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Street address |
Jin Ming Avenue
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City |
Kaifeng |
State/province |
Henan |
ZIP/Postal code |
475004 |
Country |
China |
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Platforms (1) |
GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
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Samples (12)
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Relations |
BioProject |
PRJNA177719 |