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Series GSE40614 Query DataSets for GSE40614
Status Public on Sep 05, 2013
Title Id1 knockdown in primary human glioma stem cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Abnormal activation of stemness factors is a crucial signature of cancer stem cells (CSCs), a highly tumorigenic subpopulation in malignant tumors. However, it is unclear whether multi-signaling pathways are activated in CSCs, as like normal stem cells. I would like to report that an inhibitor of differentiation 1 (ID1) activates intracellular multi-signaling involved in proliferation, genesis, and maintenance of glioma stem cells (GSCs) by suppression of Cullin3, an E3 ubiquitin ligase that degrades Cyclin E and components of SHH and WNT signaling. ID1 inhibits BMP-dependent differentiation of GSCs by activation of BMPR2-targeting miR17/20a. ID1HIGH-Cullin3LOW signature correlates with a poor prognosis of GBM patients with a significant association to gene signatures enriched in EGF, WNT, SHH, and BMP signaling. Combinational inhibition of GSC intracellular multi-signaling network increases tumor-bearing mice survival. These results provide insights on molecular and cellular basis of GSC biology, and also suggest necessity of multi-signaling inhibition for GSCs therapy.
 
Overall design Two human primary glioma stem cells (GSCs) such as GSC2 and GSC8 were isolated from two individual primary human glioma specimens. The GSCs were directly transfected with pSuper-GFP-ID1-shRNA and pSuper-GFP-Scrambled-shRNA using FuGENE 6 reagent (Roche). The RNA extraction in these cells was used to analyze gene expression.
 
Contributor(s) Jin X, Jin X
Citation(s) 27477274
Submission date Sep 05, 2012
Last update date May 14, 2017
Contact name Xiong Jin
E-mail(s) pierce0701@gmail.com
Organization name Henan University
Department School of Pharmacy
Lab Henan Key Laboratory of Brain Targeted Bio-nanomedicine
Street address Jin Ming Avenue
City Kaifeng
State/province Henan
ZIP/Postal code 475004
Country China
 
Platforms (1)
GPL6883 Illumina HumanRef-8 v3.0 expression beadchip
Samples (12)
GSM997770 GSC2_shID1_rep1
GSM997771 GSC2_shID1_rep2
GSM997772 GSC2_shID1_rep3
Relations
BioProject PRJNA177719

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40614_RAW.tar 3.9 Mb (http)(custom) TAR
GSE40614_norm_GSC2.txt.gz 7.9 Mb (ftp)(http) TXT
GSE40614_norm_GSC8.txt.gz 8.0 Mb (ftp)(http) TXT
GSE40614_raw_data.txt.gz 11.0 Mb (ftp)(http) TXT
Processed data are available on Series record
Processed data included within Sample table

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