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Series GSE40713 Query DataSets for GSE40713
Status Public on Sep 10, 2012
Title Mammary Gland Morphology and Gene Expression Signature of Prepubertal Male and Female Rats Following Exposure to Exogenous Estradiol
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary In order to properly understand whether xenoestrogens act as estrogens, it is essential to possess a solid portrait of the physiological effects of exogenous estradiol. Because the estrogen-dependent gene expression is one of the primary biomarkers of estrogenic action, we have assessed effects of three doses of exogenous estradiol (0.1, 1.0 and 10 µg/kg of body weight/day) on the mammary gland morphology and gene expression profiles by microarray analysis of prepubertal male and female rats of both sexes compared to untreated controls. Estradiol was administered subcutaneously with minipumps from weaning at PND21 to the end of the experiment at PND33. The data suggest that the male mammary is a sensitive tissue for estrogenicity assessment.
 
Overall design Groups of male and female rats were infused subcutaneously with mini-osmotic pumps with 17β-estradiol from weaning on post-natal day (PND) 21 until PND 33. Pumps delivered doses of 0, 0.1, 1.0 and 10 µg/kg/d. At sacrifice (PND 33) abdominal mammary gland pairs were collected and fresh frozen in liquid nitrogen.
 
Contributor(s) Miousse IR, Sharma N, Badeaux J, Hennings L, Shankar K, Gomez-Acevedo H, Cleves MA, Badger TM, Ronis MJ
Citation(s) 23925648, 24046281
Submission date Sep 09, 2012
Last update date Jul 31, 2017
Contact name Isabelle Racine Miousse
E-mail(s) iracinemiousse@uams.edu
Organization name UAMS
Street address 15 Children's Way
City Little Rock
ZIP/Postal code 72202
Country USA
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (24)
GSM999606 Female 0 estradiol-1
GSM999607 Female 0 estradiol-2
GSM999608 Female 0 estradiol-3
Relations
BioProject PRJNA174741

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40713_RAW.tar 63.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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