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Series GSE40839 Query DataSets for GSE40839
Status Public on Aug 05, 2013
Title Expression data from fibroblasts cultured from normal and fibrotic human lung tissue
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Pulmonary fibrosis develops as a consequence of environmentally induced lung injury and/or an inherent disease susceptibility causing fibroblast activation, proliferation and extracellular matrix deposition.
The study was undertaken to characterise global gene expression in pulmonary fibroblasts to better understand the mechanisms underlying the fibrotic fibroblast phenotype.
 
Overall design Gene expression was evaluated in lung fibroblasts derived from ten controls (normal periphery of resected tumor), open lung biopsies from eight patients with interstitial lung disease associated with systemic sclerosis (fibrotic non specific interstitial pneumonia pattern on biopsy), and from three patients with usual interstitial pneumonia. Lung fibroblasts were grown to confluence in DMEM with 10% fetal calf serum. At confluence, lung fibroblasts were serum-deprived for 44 hours in the presence of fibroblast growth medium with the addition of 0.1% bovine serum albumin (Sigma).
 
Contributor(s) Lindahl GE, Stock CJ, Shi-Wen X, Nicholson AG, Dusmet ME, Bou-Gharios G, Abraham DJ, Denton CP, Wells AU, Renzoni EA
Citation(s) 23915349
Submission date Sep 12, 2012
Last update date Aug 10, 2018
Contact name Carmel Stock
E-mail(s) c.stock@imperial.ac.uk
Organization name National Heart and Lung Institute
Department Interstitial Lung Disease
Street address 1b Manresa Road
City London
ZIP/Postal code SW3 6LR
Country United Kingdom
 
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (21)
GSM1003058 Pulmonary fibroblasts_control individual_ biological rep1
GSM1003059 Pulmonary fibroblasts_control individual_ biological rep2
GSM1003060 Pulmonary fibroblasts_control individual_ biological rep3
Relations
BioProject PRJNA175063

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40839_RAW.tar 51.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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