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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 15, 2013 |
| Title |
Expression data from mouse optic nerve head after optic nerve crush |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Reactive gliosis is a complex process that involves profound changes in gene expression. We used microarray to indentify differentially expressed genes and to investigate the molecular mechanisms of reactive gliosis in optic nerve head in response to optic nerve crush injury.
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| Overall design |
C57Bl/6 female mice were 6-8 weeks old at the time of optic nerve crush surgery. The optic nerve in the left eye was crush 1 mm behind the globe for 10 seconds and the right eye served as contralateral control. The animals were allowed to recover for 1 day, 3 day, 1 week, 3 weeks and 3 months before the optic nerve heads were collected. The naive control mice did not receive any surgery in either eye. Due to the small tissue size of the mouse optic nerve head, two optic nerve heads were pooled together for each microarray chip. The left eyes and the right eyes of two mice were combined respectively to form one pair of experiment and control samples. There were five biological replicates (10 mice) for each condition.
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| Contributor(s) |
Qu J, Jakobs TC |
| Citation(s) |
23826199 |
| Submission date |
Sep 13, 2012 |
| Last update date |
May 04, 2018 |
| Contact name |
Tatjana Jakobs |
| E-mail(s) |
tatjana_jakobs@meei.harvard.edu
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| Phone |
617-912-0205
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| Organization name |
Massachusetts Eye and Ear Infirmary
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| Department |
Howe Laboratory of Ophthalmology
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| Street address |
20 Staniford Street
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
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| Platforms (1) |
| GPL8321 |
[Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array |
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| Samples (55)
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| GSM1003387 |
optic nerve head, naïve control, biological rep1 |
| GSM1003388 |
optic nerve head, naïve control, biological rep2 |
| GSM1003389 |
optic nerve head, naïve control, biological rep3 |
| GSM1003390 |
optic nerve head, naïve control, biological rep4 |
| GSM1003391 |
optic nerve head, naïve control, biological rep5 |
| GSM1003392 |
optic nerve head, 1 day, contralateral control, biological rep1 |
| GSM1003393 |
optic nerve head, 1 day, contralateral control, biological rep2 |
| GSM1003394 |
optic nerve head, 1 day, contralateral control, biological rep3 |
| GSM1003395 |
optic nerve head, 1 day, contralateral control, biological rep4 |
| GSM1003396 |
optic nerve head, 1 day, contralateral control, biological rep5 |
| GSM1003397 |
optic nerve head, 1 day, optic nerve crushed, biological rep1 |
| GSM1003398 |
optic nerve head, 1 day, optic nerve crushed, biological rep2 |
| GSM1003399 |
optic nerve head, 1 day, optic nerve crushed, biological rep3 |
| GSM1003400 |
optic nerve head, 1 day, optic nerve crushed, biological rep4 |
| GSM1003401 |
optic nerve head, 1 day, optic nerve crushed, biological rep5 |
| GSM1003402 |
optic nerve head, 3 days, contralateral control, biological rep1 |
| GSM1003403 |
optic nerve head, 3 days, contralateral control, biological rep2 |
| GSM1003404 |
optic nerve head, 3 days, contralateral control, biological rep3 |
| GSM1003405 |
optic nerve head, 3 days, contralateral control, biological rep4 |
| GSM1003406 |
optic nerve head, 3 days, contralateral control, biological rep5 |
| GSM1003407 |
optic nerve head, 3 days, optic nerve crushed, biological rep1 |
| GSM1003408 |
optic nerve head, 3 days, optic nerve crushed, biological rep2 |
| GSM1003409 |
optic nerve head, 3 days, optic nerve crushed, biological rep3 |
| GSM1003410 |
optic nerve head, 3 days, optic nerve crushed, biological rep4 |
| GSM1003411 |
optic nerve head, 3 days, optic nerve crushed, biological rep5 |
| GSM1003412 |
optic nerve head, 1 week, contralateral control, biological rep1 |
| GSM1003413 |
optic nerve head, 1 week, contralateral control, biological rep2 |
| GSM1003414 |
optic nerve head, 1 week, contralateral control, biological rep3 |
| GSM1003415 |
optic nerve head, 1 week, contralateral control, biological rep4 |
| GSM1003416 |
optic nerve head, 1 week, contralateral control, biological rep5 |
| GSM1003417 |
optic nerve head, 1 week, optic nerve crushed, biological rep1 |
| GSM1003418 |
optic nerve head, 1 week, optic nerve crushed, biological rep2 |
| GSM1003419 |
optic nerve head, 1 week, optic nerve crushed, biological rep3 |
| GSM1003420 |
optic nerve head, 1 week, optic nerve crushed, biological rep4 |
| GSM1003421 |
optic nerve head, 1 week, optic nerve crushed, biological rep5 |
| GSM1003422 |
optic nerve head, 3 weeks, contralateral control, biological rep1 |
| GSM1003423 |
optic nerve head, 3 weeks, contralateral control, biological rep2 |
| GSM1003424 |
optic nerve head, 3 weeks, contralateral control, biological rep3 |
| GSM1003425 |
optic nerve head, 3 weeks, contralateral control, biological rep4 |
| GSM1003426 |
optic nerve head, 3 weeks, contralateral control, biological rep5 |
| GSM1003427 |
optic nerve head, 3 weeks, optic nerve crushed, biological rep1 |
| GSM1003428 |
optic nerve head, 3 weeks, optic nerve crushed, biological rep2 |
| GSM1003429 |
optic nerve head, 3 weeks, optic nerve crushed, biological rep3 |
| GSM1003430 |
optic nerve head, 3 weeks, optic nerve crushed, biological rep4 |
| GSM1003431 |
optic nerve head, 3 weeks, optic nerve crushed, biological rep5 |
| GSM1003434 |
optic nerve head, 3 months, contralateral control, biological rep1 |
| GSM1003435 |
optic nerve head, 3 months, contralateral control, biological rep2 |
| GSM1003436 |
optic nerve head, 3 months, contralateral control, biological rep3 |
| GSM1003437 |
optic nerve head, 3 months, contralateral control, biological rep4 |
| GSM1003438 |
optic nerve head, 3 months, contralateral control, biological rep5 |
| GSM1003439 |
optic nerve head, 3 months, optic nerve crushed, biological rep1 |
| GSM1003440 |
optic nerve head, 3 months, optic nerve crushed, biological rep2 |
| GSM1003441 |
optic nerve head, 3 months, optic nerve crushed, biological rep3 |
| GSM1003442 |
optic nerve head, 3 months, optic nerve crushed, biological rep4 |
| GSM1003443 |
optic nerve head, 3 months, optic nerve crushed, biological rep5 |
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| Relations |
| BioProject |
PRJNA175121 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40857_RAW.tar |
130.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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