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| Status |
Public on Sep 15, 2012 |
| Title |
Data expression in alveolar macrophages induced by lipopolysaccharide in humans |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Rationale: Lipopolysaccharide (LPS) is ubiquitous in the environment. Inhalation of LPS has been implicated in the pathogenesis and/or severity of several lung diseases, including pneumonia, chronic obstructive pulmonary disease and asthma. Alveolar macrophages are the main resident leukocytes exposed to inhaled antigens. Objectives: To obtain insight into which innate immune pathways become activated within human alveolar macrophages upon exposure to LPS in vivo.
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| Overall design |
In seven healthy humans sterile saline was instilled into a lung segment by bronchoscope, followed by instillation of LPS into the contralateral lung. Six hours later a bilateral bronchoalveolar lavage was performed and whole-genome transcriptional profiling was done (Affymetrix HG-U133 Plus 2.0) on purified alveolar macrophages, comparing cells exposed to saline or LPS from the same individuals.
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| Contributor(s) |
Reynier F, de Vos AF, Hoogerwerf JJ, Bresser P, van der Zee JS, Paye M, Pachot A, Mougin B, van der Poll T |
| Citation(s) |
22952057 |
| Submission date |
Sep 14, 2012 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Frederic Reynier |
| E-mail(s) |
frederic.reynier@biomerieux.com
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| Organization name |
bioMerieux
|
| Street address |
Chemin de l'Orme
|
| City |
Marcy L'Etoile |
| ZIP/Postal code |
69280 |
| Country |
France |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (14)
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| GSM1004101 |
Subject 1 / lung subsegment instillated by Saline |
| GSM1004102 |
Subject 1 / lung subsegment instillated by LPS |
| GSM1004103 |
Subject 2 / lung subsegment instillated by Saline |
| GSM1004104 |
Subject 2 / lung subsegment instillated by LPS |
| GSM1004105 |
Subject 3 / lung subsegment instillated by Saline |
| GSM1004106 |
Subject 3 / lung subsegment instillated by LPS |
| GSM1004107 |
Subject 4 / lung subsegment instillated by Saline |
| GSM1004108 |
Subject 4 / lung subsegment instillated by LPS |
| GSM1004109 |
Subject 5 / lung subsegment instillated by Saline |
| GSM1004110 |
Subject 5 / lung subsegment instillated by LPS |
| GSM1004111 |
Subject 6 / lung subsegment instillated by Saline |
| GSM1004112 |
Subject 6 / lung subsegment instillated by LPS |
| GSM1004113 |
Subject 7 / lung subsegment instillated by Saline |
| GSM1004114 |
Subject 7 / lung subsegment instillated by LPS |
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| Relations |
| BioProject |
PRJNA175261 |
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