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| Status |
Public on Dec 31, 2013 |
| Title |
Targets of the bHLH transcription factor, Sage |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
FoxA transcription factors play major roles in organ-specific gene expression. How FoxA proteins achieve specificity is unclear, given their broad expression patterns and requirements in multiple cell types. Here, we characterize Sage, a basic helix-loop-helix (bHLH) transcription factor expressed exclusively in the Drosophila salivary gland (SG). We identify Sage targets and show that not only are both Sage and the single Drosophila FoxA protein, Fork head (Fkh), required for expression of these genes, but coexpression of Sage and Fkh is sufficient to drive target gene expression in multiple other cell types. Sage and Fkh drive expression of the bZip transcription factor Senseless (Sens), which boosts expression of Sage/Fkh targets. Importantly, Sage, Fkh and Sens colocalize on salivary gland polytene chromosomes. Thus, Fkh drives cell-type specific gene expression as part of a tissue-specific transcription module that includes Sage and Sens, providing a new paradigm for how mammalian FoxA proteins acheive specificity.
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| Overall design |
Three wild type (reference) samples were obtained from the Oregon R strain, age matched and treated the same as the three experimental samples isolated from Stages 11-16 sage mutant embryos.
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| Contributor(s) |
Fox R, Andrew DJ |
| Citation(s) |
32613751 |
| Submission date |
Sep 18, 2012 |
| Last update date |
Jun 03, 2021 |
| Contact name |
Deborah J Andrew |
| E-mail(s) |
dandrew@jhmi.edu
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| Organization name |
Johns Hopkins University School of Medicine
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| Department |
Cell Biology
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| Street address |
725 N. Wolfe St., Hunterian G-1
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA175439 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40963_RAW.tar |
11.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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