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| Status |
Public on Jan 01, 2013 |
| Title |
Gene expression profile of the developing human retinal pigment epithelium |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The microarray technique was used to investigate gene expression level changes in human retinal pigment epithelium (RPE) microdissected from fetal eyes (13 and 16 weeks of gestation) and adult eyes (40-60 years old). The gene expression analysis of human fetal RPE during development were performed and compared to human native RPE.
Of the 45,033 probe sets on the microarray, 30,736 were detected. 3498 differentially expressed genes could be clustered into 8 patterns of expression that were statistically significant. Analyzing expression pattern of genes coding for key functions (pigment synthesis, visual cycle, phagocytosis, adherens and tight junction, transcellular transport) indicates human RPE achieved a high degree of maturity at early pregnancy. Compare to 154 signature genes of RPE, 148 candidate genes were identified in this studies including 53 down-regulated genes and 5 up-regulated genes. qRT2-PCR results showed similar expression trends with the microarray at three time points.These findings indicate human RPE has different expression pattern compared to other animals.
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| Overall design |
A three chip study using total RNA pooled equally from three 13-week fetal eyes, two 16-week fetal eyes and two adult eyes for three time points (13, 16 week gestation and mature adult eye). Experiment was repeated in another group samples. This is a six chip study totally.
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| Contributor(s) |
Zhang Z, Zhang Y, Peng S |
| Citation(s) |
23487591 |
| Submission date |
Sep 19, 2012 |
| Last update date |
May 28, 2014 |
| Contact name |
Shaomin Peng |
| E-mail(s) |
psm06511@yahoo.com
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| Organization name |
The Second Hospital of Harbin Medical University
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| Street address |
246 XueFu Road
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| City |
Harbin |
| ZIP/Postal code |
150086 |
| Country |
China |
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| Platforms (2) |
| GPL10191 |
NimbleGen Homo sapiens HG18 090828 opt expr HX12 (12x135k) |
| GPL16025 |
NimbleGen Homo sapiens Expression Array [100718_HG18_opt_expr] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA175482 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40980_RAW.tar |
58.0 Mb |
(http)(custom) |
TAR (of CALLS, PAIR) |
| GSE40980_RPE_13W_vs_Adult_rep1.txt.gz |
1.8 Mb |
(ftp)(http) |
TXT |
| GSE40980_RPE_13W_vs_Adult_rep2.txt.gz |
2.3 Mb |
(ftp)(http) |
TXT |
| GSE40980_RPE_16W_vs_13W_rep1.txt.gz |
399.0 Kb |
(ftp)(http) |
TXT |
| GSE40980_RPE_16W_vs_13W_rep2.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE40980_RPE_16W_vs_Adult_rep1.txt.gz |
1.8 Mb |
(ftp)(http) |
TXT |
| GSE40980_RPE_16W_vs_Adult_rep2.txt.gz |
2.3 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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