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Series GSE40994 Query DataSets for GSE40994
Status Public on Sep 20, 2012
Title Molecular profiling of activated neurons by phosphorylated ribosome capture [Illumina BeadArray]
Organism Mus musculus
Experiment type Expression profiling by array
Summary We report that phosphorylated ribosomes can be immunoprecipitated from mouse brain homogenates, enriching for mRNAs that were selectively expressed in activated neurons
 
Overall design Mice were given an injection of concentrated salt solution (2M NaCl, 325 uL) or PBS, and then sacrificed 2h later and hypothalami dissected. Tissue homogenates were prepared from hypothalamus and phosphorylated ribosomes immunoprecipitated using pS6 244/247 antibodies. The immunoprecipitated RNA and total RNA from both control and salt treated animals were then purified and analyzed by Illumina Microarray.
 
Contributor(s) Knight ZA, Friedman JM
Citation(s) 23178128
Submission date Sep 19, 2012
Last update date Jun 14, 2018
Contact name Zachary Knight
Organization name Rockefeller University
Street address 1230 York Ave
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (4)
GSM1006489 Salt IP/Input #1
GSM1006490 Control IP/Input #1
GSM1006491 Salt IP/Input #2
This SubSeries is part of SuperSeries:
GSE40995 Molecular profiling of activated neurons by phosphorylated ribosome capture
Relations
BioProject PRJNA175511

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE40994_RAW.tar 3.1 Mb (http)(custom) TAR
GSE40994_merged_ratio_matrix.txt.gz 2.8 Mb (ftp)(http) TXT
GSE40994_non-normalized.txt.gz 1.6 Mb (ftp)(http) TXT
GSE40994_readme.txt 162 b (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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