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Series GSE41020 Query DataSets for GSE41020
Status Public on Sep 25, 2012
Title Sca-1 is an early-response target of histone deacetylase inhibitors and marks hematopoietic cells with enhanced function
Organism Mus musculus
Experiment type Expression profiling by array
Summary Expression profiles of LSK cells stimulated for 24h in the presence or in the absence of of valproic acid (VPA)
The molecular process that underlies the biological effects of valproic acid (VPA), a widely used histone deacetylase inhibitor, on HSPCs was investigated by studying the early-response genes of VPA. Genome-wide gene expression studies revealed overrepresentation of genes involved in glutathione metabolism, receptor and signal transducer activity and changes in the HSPCs surface profile following short, 24h VPA treatment. Sca-1, a well-known and widely used stem cell surface marker, was identified as a prominent VPA target.VPA strongly preserved Sca-1 expression on LSK cells, but also re-activated Sca-1 on committed progenitor cells that were Sca-1 negative, thereby reverting them to the LSK phenotype. We demonstrated that re-acquired Sca-1 expression coincided with induced self-renewal capacity as measured by in vitro re-plating assays, while Sca-1 itself was not required for the biological effects of VPA as demonstrated using Sca-1 deficient progenitor cells. We show that VPA can induce several genes involved in signal transduction of which Sca-1 was shown to mark cells with increased self-renewal capacity.
 
Overall design These data consist of total mRNA obtained from hematopoietic cells cultured for 24h in the presence or absence of valproic acid. All samples were analyzed in independent biological triplicates.
 
Contributor(s) Walasek MA, Bystrykh LV, Olthof S, de Haan G, van Os R
Citation(s) 22989761
Submission date Sep 20, 2012
Last update date Jun 14, 2018
Contact name Leonid Bystrykh
E-mail(s) l.bystrykh@umcg.nl
Organization name University Medical Center Groningen
Department European Research Institute for the Biology of Ageing - ERIBA
Lab Ageing Biology and Stem Cells
Street address A. Deusinglaan 1
City Groningen
State/province Groningen
ZIP/Postal code 9713 AV
Country Netherlands
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (6)
GSM1006896 Control-non-(VPA)-stimulated_rep1
GSM1006897 Control-non-(VPA)-stimulated_rep2
GSM1006898 Control-non-(VPA)-stimulated_rep3
Relations
BioProject PRJNA175906

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41020_RAW.tar 3.1 Mb (http)(custom) TAR
GSE41020_non-normalized_data.txt.gz 1.0 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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