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| Status |
Public on Feb 28, 2013 |
| Title |
Expression profiles of zebrafish transgenic line Tg(fabp10a:def)-I |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
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| Summary |
We previously reported that Def (Digestive-organ expansion factor) was a pan-endodermal enriched factor that is essential for the growth of digestive organs in zebrafish using a def mutant line hi429 as model (Chen et al., 2005). To further elucidate Def function, we generated a Def over-expressed zebrafish line, namely Tg (fabp10a:def)-I, in which def expression was under the control of a liver-specific promoter fabp10a.
We used microarrays to detail the global programme of gene expression in Tg (fabp10a:def)-I transgenic line compared with wild type zebrafish control, and identified distinct classes of differently regulated genes in Tg (fabp10a:def)-I line.
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| Overall design |
Total RNA from the adult livers of three independent batches of 3-month-old Tg(fabp10a:def)-I and wild type (AB strain) male fish was extracted using TRIzol (Invitrogen), each batch with the liver samples from three fish mixed together. The samples were subjected to microarray hybridization and the data were generated for further analysis.
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| Contributor(s) |
Tao T, Shi H, Peng J |
| Citation(s) |
23593122 |
| Submission date |
Sep 21, 2012 |
| Last update date |
Jan 25, 2018 |
| Contact name |
Jinrong Peng |
| E-mail(s) |
pengjr@zju.edu.cn
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| Organization name |
Zhejiang University
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| Street address |
866 Yuhangtang Road
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| City |
Hangzhou |
| ZIP/Postal code |
310029 |
| Country |
China |
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| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
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| Samples (6)
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| GSM1008113 |
wild type zebrafish liver_biological rep1 |
| GSM1008114 |
wild type zebrafish liver_biological rep2 |
| GSM1008115 |
wild type zebrafish liver_biological rep3 |
| GSM1008116 |
Tg(fabp10a:def)-I liver_biological rep1 |
| GSM1008117 |
Tg(fabp10a:def)-I liver_biological rep2 |
| GSM1008118 |
Tg(fabp10a:def)-I liver_biological rep3 |
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| Relations |
| BioProject |
PRJNA175641 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE41059_RAW.tar |
9.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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