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Series GSE41059 Query DataSets for GSE41059
Status Public on Feb 28, 2013
Title Expression profiles of zebrafish transgenic line Tg(fabp10a:def)-I
Organism Danio rerio
Experiment type Expression profiling by array
Summary We previously reported that Def (Digestive-organ expansion factor) was a pan-endodermal enriched factor that is essential for the growth of digestive organs in zebrafish using a def mutant line hi429 as model (Chen et al., 2005). To further elucidate Def function, we generated a Def over-expressed zebrafish line, namely Tg (fabp10a:def)-I, in which def expression was under the control of a liver-specific promoter fabp10a.
We used microarrays to detail the global programme of gene expression in Tg (fabp10a:def)-I transgenic line compared with wild type zebrafish control, and identified distinct classes of differently regulated genes in Tg (fabp10a:def)-I line.
 
Overall design Total RNA from the adult livers of three independent batches of 3-month-old Tg(fabp10a:def)-I and wild type (AB strain) male fish was extracted using TRIzol (Invitrogen), each batch with the liver samples from three fish mixed together. The samples were subjected to microarray hybridization and the data were generated for further analysis.
 
Contributor(s) Tao T, Shi H, Peng J
Citation(s) 23593122
Submission date Sep 21, 2012
Last update date Jan 25, 2018
Contact name Jinrong Peng
E-mail(s) pengjr@zju.edu.cn
Organization name Zhejiang University
Street address 866 Yuhangtang Road
City Hangzhou
ZIP/Postal code 310029
Country China
 
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (6)
GSM1008113 wild type zebrafish liver_biological rep1
GSM1008114 wild type zebrafish liver_biological rep2
GSM1008115 wild type zebrafish liver_biological rep3
Relations
BioProject PRJNA175641

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41059_RAW.tar 9.7 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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