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Series GSE41086 Query DataSets for GSE41086
Status Public on Jan 01, 2013
Title Gene expression profiling of Nodal versus Activin-derived endoderm from mouse ES cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary To explore the molecular basis of functional differences observed between Nodal versus Activin-derived endoderm, we compared their respective gene expression profiles. Sox17-GFP mouse ES cells were differentiated in the presence of Nodal or Activin for 7 days, after which GFP(+) cells were purified by FACs. Undifferentiated ES cells were also included for comparison as a control. Results indicate that the two endoderm populations are nearly identical at the level of global transcription. Subtle differences suggest a difference in the degree of endoderm progression.
Overall design Total RNA obtained from SOX17-GFP (+) mouse ES cells, Sox17-GFP treated with Nodal, Sox17-GFP treated with Activin.
Two replicates for each sample; Sample data table contains average values of two replicates. The non_normalized.txt file contains individual signal values for each replicate.
Contributor(s) Chen AE, Melton DA
Citation(s) 23293299
Submission date Sep 22, 2012
Last update date Jun 14, 2018
Contact name Alice E Chen
Organization name Harvard University
Department Stem Cell and Regenerative Biology
Lab Douglas Melton
Street address 7 Divinity Avenue
City Cambridge
State/province Ma
ZIP/Postal code 02138
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (3)
GSM1008437 Sox17-GFP(+) from Activin treatment
GSM1008438 Sox17-GFP(+) from Nodal treatment
GSM1008439 undifferentiated Sox17-GFP mES cells
BioProject PRJNA175712

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41086_RAW.tar 3.1 Mb (http)(custom) TAR
GSE41086_non_normalized.txt.gz 1.1 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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