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Series GSE41089 Query DataSets for GSE41089
Status Public on Nov 20, 2012
Title Expression data from hearts of wild-type C57BL/6 mice infected with T. cruzi and controls (uninfected)
Organism Mus musculus
Experiment type Expression profiling by array
Summary An efficient innate immune recognition of the intracellular parasite T. cruzi is crucial for host protection against development of Chagas disease, which often leads to multiple organ damage, particularly the heart leading to cardiomyopathy. Mechanisms modulated by MyD88 have been shown to be necessary for resistance against T, cruzi infection. Recently, Nod-like receptors have been shown to play an important role as innate immune sensors, particularly as they relate to inflammasome function, caspase activation, and inflammatory cytokine production. In this study, we aimed to investigate the participation of innate immune responses in general, and inflammasomes in particular, in heart inflammation and cardiac damage upon infection with the T. cruzi parasite.
We used microarrays to gain insight into gene expression in the cardiac tissue of mice infected with the causative agent of Trypanosoma cruzi, and identified distinct classes of up-regulated genes during this process, including important genes involved in inflammasome activation and innate immune responses in general.
 
Overall design The hearts of C57BL/6 mice day 18 post-infection with a Y strain of the parasite T. cruzi, and uninfected controls were extraced for RNA extraction and hybridization on Affymetrix microarrays. We sought to compare gene expression among two groups of mice, and so extracted the hearts of 3 control uninfected mice, and of 3 infected mice 18 days post-infection.
 
Contributor(s) De Niz MI, Franklin BS, Gazzinelli RT
Citation(s) 23966627
Submission date Sep 22, 2012
Last update date May 04, 2018
Contact name Ricardo Gazzinelli
Organization name University of Massachussetts Medical School
Department Infectious Diseases and Immunology
Street address 364 Plantation Street, LRB
City Worcester MA
ZIP/Postal code 01605
Country USA
 
Platforms (1)
GPL8321 [Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array
Samples (6)
GSM1008462 C57BL6_Infected1
GSM1008463 C57BL6_Infected2
GSM1008464 C57BL6_Infected3
Relations
BioProject PRJNA175735

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41089_Genes_significantly_expressed.xls.gz 887.3 Kb (ftp)(http) XLS
GSE41089_RAW.tar 56.2 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

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