|
| Status |
Public on Oct 18, 2012 |
| Title |
Expression data from uninfected and VSV-infected Drosophila cells at 4 hours post-infection |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
Expression profiling of rapidly-induced genes upon VSV infection at 4 hours post-infection in Drosophila cells
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| Overall design |
Drosophila cells were treated with Bgal dsRNA and either treated as uninfected or infected with VSV (MOI 10) for 4 hours, in two independent biological replicates. Total RNA was isolated using Trizol and microarray experiments were performed at the University of Pennsylvania Microarray Facility (U. Penn MF) following directions according to the manufacturer’s protocol (Affymetrix). In brief, 100 ng RNA were amplified with the Ovation RNA Amplification System v2 (NuGen), the Encore Biotin Module (NuGen) was used for fragmentation and labeling, Protocol FS450 002 was used for hybridization, washing, and staining. Images were scanned using the GeneChip® Scanner 3000 and image analysis was performed using the Affymetrix® GeneChip® Command Console® Software (AGCC).
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| Contributor(s) |
Xu J, Tudor M, Cherry S |
| Citation(s) |
23084920 |
| Submission date |
Sep 25, 2012 |
| Last update date |
May 04, 2018 |
| Contact name |
Sara Cherry |
| E-mail(s) |
cherrys@mail.med.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Street address |
304K Lynch Labs
|
| City |
Philadelphia |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
|
| Samples (4)
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| GSM1009313 |
Bgal dsRNA at T4 hours, biological rep1 |
| GSM1009314 |
Bgal dsRNA at T4 hours, biological rep2 |
| GSM1009315 |
Bgal dsRNA and VSV infection at T4 hours, biological rep1 |
| GSM1009316 |
Bgal dsRNA and VSV infection at T4 hours, biological rep2 |
|
| Relations |
| BioProject |
PRJNA175918 |
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