|
| Status |
Public on Nov 01, 2012 |
| Title |
Expression Profiles of miRNAs in brown fat of mice at room temperature and cold exposure (8C) |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
microRNAs levels were measured in brown adipose tissue of male C57Bl6N mice that were kept at RT or during cold exposure (8°C) for 24 hrs. Several miRNAs including myomirs were identified to be regulated in response to cold.
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| |
|
| Overall design |
Brown adipose tissue from 2 groups of 8 weeks old male C57Bl6N mice at room temperature (n=3) or after cold exposure n=3) at 8°C for 24 hrs were excised and RNA was extracted using Trizol and the manufactureres recommended procedure. miRNA expression levels were determined using Agilent microRNA expression analysis platform.
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| |
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| Contributor(s) |
von Meyenn F, Stoffel M |
| Citation(s) |
23143398 |
| Submission date |
Oct 03, 2012 |
| Last update date |
Jul 11, 2014 |
| Contact name |
Markus Stoffel |
| E-mail(s) |
stoffel@biol.ethz.ch
|
| Organization name |
ETH Zurich
|
| Department |
Molecular Health Science
|
| Lab |
Metabolic Diseases
|
| Street address |
Schafmattstr. 22
|
| City |
Zurich |
| State/province |
ZH |
| ZIP/Postal code |
8093 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL7732 |
Agilent-019119 Mouse miRNA Microarray 1.0 G4472A |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA178548 |
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