|
| Status |
Public on Oct 04, 2012 |
| Title |
BG3 WT Repliseq |
| Project |
modENCODE
|
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
|
| Summary |
modENCODE_submission_5528
This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648
Project Goal: The relative time of replication for all unique sequences in the Drosophila genome was determined by sequencing replication intermediates that were differentially labeled with BrdU during S-phase progression. Briefly, cells were pulse labeled with BrdU and sorted into early, middle and late S-phase fractions. BrdU labeled sequences from each fraction of S-phase were enriched by immunoprecipitation and sequenced on the Illumina platform.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
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| |
|
| Overall design |
EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Cell Line: ML-DmBG3-c2; Tissue: CNS-derived cell-line; Developmental Stage: third instar larval stage; Genotype: y v f mal; Sex: Unknown; EXPERIMENTAL FACTORS: read length (base pair) ; Cell Line ML-DmBG3-c2
|
| Web link |
http://www.modencode.org
http://0-www-ncbi-nlm-nih-gov.brum.beds.ac.uk/geo/info/ENCODE.html
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| |
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| Contributor(s) |
MacAlpine D, Lubelsky Y, Belsky J, Prinz J |
| Citation(s) |
24985913 |
| BioProject |
PRJNA63463 |
| Submission date |
Oct 04, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
DCC modENCODE |
| E-mail(s) |
help@modencode.org
|
| Phone |
416-673-8579
|
| Organization name |
Ontario Institute for Cancer Research
|
| Lab |
modENCODE DCC
|
| Street address |
MaRS Centre, South Tower, 101 College Street, Suite 800
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 0A3 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
|
| Samples (8)
|
| GSM1015358 |
ML-DmBG3-c2 WT Early S-phase Replicate 1 extraction1_seq1 aliquote 1 |
| GSM1015359 |
ML-DmBG3-c2 WT Mid-Early S-phase Replicate 1 extraction2_seq1 aliquote 1 |
| GSM1015360 |
ML-DmBG3-c2 WT Mid-Late S-phase Replicate 1 extraction3_seq1 aliquote 1 |
| GSM1015361 |
ML-DmBG3-c2 WT LateSphase Replicate 1 extraction4_seq1 aliquote 1 |
| GSM1015362 |
ML-DmBG3-c2 WT Early S-phase Replicate 2 extraction5_seq1 aliquote 1 |
| GSM1015363 |
ML-DmBG3-c2 WT Mid-Early S-phase_Replicate 2 extraction6_seq1 aliquote 1 |
| GSM1015364 |
ML-DmBG3-c2 WT Mid-Late S-phase Replicate 2 extraction7_seq1 aliquote 1 |
| GSM1015365 |
ML-DmBG3-c2 WT Late S-phase Replicate 2 extraction8_seq1 aliquote 1 |
|
| Relations |
| SRA |
SRP016083 |
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