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Series GSE4142 Query DataSets for GSE4142
Status Public on Feb 02, 2006
Title Molecular Analysis of antigen-specific B cell responses
Organism Mus musculus
Experiment type Expression profiling by array
Summary In order to better understand the factors that regulate B cell differentiation upon exposure to antigen, we compares global gene expression profiles from naive B cells with antigen-specific plasma, germinal center, and memory B cells after immunization with the T-dependent antigen, NP-CGG. The memory B cell-enriched transcripts were then compared with memory T cell-enriched and hematopoietic stem cell-enriched transcripts in order to generate a transcriptional profile of self-renewal within the hematopoietic system.
Keywords: Cell Type Comparison
 
Overall design Naive B cells were FACS sorted from unimmunized mice, while plasma, germinal center, and memory B cells were sorted from NP-CGG-immunized mice at 1, 2, or 10 weeks after immunization, respectively, using a combination of cell surface markers. RNA was extracted, amplified, and hybridized to Affymetrix microarrays.
 
Contributor(s) Bhattacharya D, Weissman IL, Luckey CJ, Benoist C, Mathis D
Citation(s) 16492737, 17982071
Submission date Jan 31, 2006
Last update date Jul 31, 2019
Contact name Irving L. Weissman
E-mail(s) irv@stanford.edu, deepta@stanford.edu
Phone 6507235278
Fax 6507234034
Organization name Stanford University
Street address 279 Campus Dr.
City Stanford
State/province CA
ZIP/Postal code 94305-5323
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (14)
GSM94741 Naive B cell replicate 1
GSM94744 Naive B cell replicate 2
GSM94745 Naive B cell replicate 3
Relations
BioProject PRJNA94991

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4142_RAW.tar 80.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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