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Series GSE4142 Query DataSets for GSE4142
Status Public on Feb 02, 2006
Title Molecular Analysis of antigen-specific B cell responses
Organism Mus musculus
Experiment type Expression profiling by array
Summary In order to better understand the factors that regulate B cell differentiation upon exposure to antigen, we compares global gene expression profiles from naive B cells with antigen-specific plasma, germinal center, and memory B cells after immunization with the T-dependent antigen, NP-CGG. The memory B cell-enriched transcripts were then compared with memory T cell-enriched and hematopoietic stem cell-enriched transcripts in order to generate a transcriptional profile of self-renewal within the hematopoietic system.
Keywords: Cell Type Comparison
Overall design Naive B cells were FACS sorted from unimmunized mice, while plasma, germinal center, and memory B cells were sorted from NP-CGG-immunized mice at 1, 2, or 10 weeks after immunization, respectively, using a combination of cell surface markers. RNA was extracted, amplified, and hybridized to Affymetrix microarrays.
Contributor(s) Bhattacharya D, Weissman IL, Luckey CJ, Benoist C, Mathis D
Citation(s) 16492737, 17982071
Submission date Jan 31, 2006
Last update date Jul 31, 2019
Contact name Irving L. Weissman
Phone 6507235278
Fax 6507234034
Organization name Stanford University
Street address 279 Campus Dr.
City Stanford
State/province CA
ZIP/Postal code 94305-5323
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (14)
GSM94741 Naive B cell replicate 1
GSM94744 Naive B cell replicate 2
GSM94745 Naive B cell replicate 3
BioProject PRJNA94991

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Supplementary file Size Download File type/resource
GSE4142_RAW.tar 80.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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