|
| Status |
Public on Feb 01, 2013 |
| Title |
Histone H3K9 Methyltransferase G9a Represses PPARγ Expression and Adipogenesis [ChIP-Seq and RNA-Seq data] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
PPARγ promotes adipogenesis while Wnt proteins inhibit adipogenesis. However, the mechanisms that control expression of these positive and negative master regulators of adipogenesis remain incompletely understood. By genome-wide histone methylation profiling in preadipocytes, we find that among gene loci encoding adipogenesis regulators, histone methyltransferase (HMT) G9a-mediated repressive epigenetic mark H3K9me2 is enriched on the entire PPARγ locus. H3K9me2 and G9a levels decrease during adipogenesis, which correlates inversely with induction of PPARγ. Removal of H3K9me2 by G9a deletion enhances chromatin opening and binding of adipogenic transcription factor C/EBP-beta to PPARγ promoter, which promotes PPARγ expression. Interestingly, G9a represses PPARγ expression in an HMT activity-dependent manner but facilitates Wnt10a expression independent of its enzymatic activity. Consistently, deletion of G9a or inhibiting G9a HMT activity promotes adipogenesis. Finally, deletion of G9a in mouse adipose tissues increases adipogenic gene expression and tissue weight. Thus, by inhibiting PPARγ expression and facilitating Wnt10a expression, G9a represses adipogenesis.
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| |
|
| Overall design |
Examination of 3 different histone modification changes in 3T3-L1 preadipocytes
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| |
|
| Contributor(s) |
Wang L, Xu S, Ge K |
| Citation(s) |
23178591 |
| Submission date |
Oct 10, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Kai Ge |
| E-mail(s) |
kai.ge@nih.gov
|
| Phone |
301-451-1998
|
| Organization name |
NIH
|
| Department |
NIDDK
|
| Street address |
10 Center Dr Rm 8N307
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE41457 |
Histone H3K9 Methyltransferase G9a Represses PPARγ Expression and Adipogenesis |
|
| Relations |
| BioProject |
PRJNA177076 |
| SRA |
SRP016054 |
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