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Status |
Public on Oct 11, 2012 |
Title |
Expression data from Caenorhabditis elegans exposed to single-walled carbon nanotubes |
Organism |
Caenorhabditis elegans |
Experiment type |
Expression profiling by array
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Summary |
The potential environmental risk of single-walled carbon nanotubes (SWCNTs) is evaluated using Caenorhabditis elegans (C. elegans) as an ecotoxicological animal model. Highly soluble amide-modified SWCNTs (a-SWCNTs) are used in the present study so that the dose-response impact of SWCNTs could be studied. mechanisms. a-SWCNTs are efficiently taken up by worms during feeding and cause significant toxicity in worms, including retarded growth, shortened lifespan and defective embryogenesis. Genome-wide gene expression analysis is performed to investigate the toxic molecular
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Overall design |
We examined the effect of different concentrations of a-SWCNTs (0, 100, 250 and 500 μg/mL) on the growth of C. elegans. We measured the body length of worms reaching the L4 stage after a-SWCNT exposure for 48 hr at 22°C. Compared to the untreated worms, we found that the average length of worms exposed to a-SWCNTs (500 μg mL-1) was significantly shorter than the untreated groups. In addition, the dose of a-SWCNTs also caused retarded growth, reduced lifespan and defective embryogenesis in worms. Genome-wide gene expression analysis using an Affymetrix GeneChip was performed to further investigate the molecular basis of these defects.
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Contributor(s) |
Chou C, Hsiao K, Chen P |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
Submission date |
Oct 10, 2012 |
Last update date |
Jul 06, 2016 |
Contact name |
Cheng Chung Chou |
E-mail(s) |
cyberman@ms1.url.com.tw
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Organization name |
ChungCheng university
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Street address |
168 University Road, Minhsiung Township
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City |
Chiayi |
State/province |
International |
ZIP/Postal code |
62102 |
Country |
Taiwan |
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Platforms (1) |
GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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Samples (2) |
GSM1018113 |
C. elegans grown without a-SWCNT treatment for 48 hr at 22°C. |
GSM1018114 |
C. elegans exposed to 500 μg/mL of a-SWCNT for 48 hr at 22°C. |
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Relations |
BioProject |
PRJNA177179 |
Supplementary file |
Size |
Download |
File type/resource |
GSE41486_RAW.tar |
4.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
Processed data included within Sample table |
Processed data provided as supplementary file |
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