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Series GSE41521 Query DataSets for GSE41521
Status Public on Mar 27, 2013
Title Genome wide analysis of C57BL-6 mice infected with European strain (P1/7) of Streptococcus suis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Streptococcus suis is a major swine pathogen that can be transmitted to humans causing severe symptoms. A large human outbreak was described in China, where approximately 25% out of 215 infected humans developed an unusual streptococcal toxic shock-like syndrome (STSLS). Albeit increased expression of inflammatory mediators following infection by the Chinese S. suis strain was suggested as responsible for STSLS case severity, the mechanisms involved are still poorly understood. In this study, we investigated the host innate immune response to infection by either one of 3 strains of S. suis: 89-1591 (Canadian, intermediate virulence), P1/7 (European, high virulence), and SC84 (Chinese, epidemic strain). Using Illumina microarray and validating those results with qPCR and Luminex assay, infected mice showed elevated expression of mainly pro-inflammatory chemokine and cytokine genes. Generally, pro-inflammatory genes were expressed at a higher level in mice infected with S. suis strain SC84 > P1/7 > 89-1591. Interestingly, IFNγ was expressed at much higher levels only in mice infected with the S. suis strain SC84, which could potentially explain some of the STSLS symptoms. IFNγ-KO mice infected with SC84 showed better survival than WT mice while no differences was seen in mice infected with highly virulent P1/7 strain. Overall, our results show an important role of IFNγ in S. suis infections and might explain in part the increased virulence of SC84 responsible for a recent outbreak in China.
 
Overall design Total RNA obtained from spleen of C57BL/6 mice infected with Streptococcus suis strain P1/7 or mock-infected with THB. Four replicates in both groups.
 
Contributor(s) Lachance C, Gottschalk M, Gerber PP, Lemire P, Xu J, Segura M
Citation(s) 23509145
Submission date Oct 11, 2012
Last update date Jun 14, 2018
Contact name Claude Lachance
E-mail(s) claude.lachance@mail.mcgill.ca
Organization name Bio-Rad
Street address 1329 Meyerside Drive
City Mississauga
State/province Ontario
ZIP/Postal code L5T 1C9
Country Canada
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (8)
GSM1018515 Spleen Mock infected 6h Rep 1 (P1/7)
GSM1018516 Spleen Mock infected 6h Rep 2 (P1/7)
GSM1018517 Spleen Mock infected 6h Rep 3 (P1/7)
Relations
BioProject PRJNA177348

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41521_RAW.tar 3.1 Mb (http)(custom) TAR
GSE41521_non-normalized_P1_7.txt.gz 1.3 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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