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Series GSE41607 Query DataSets for GSE41607
Status Public on Oct 18, 2013
Title Gene expression data in Mouse Lung Epithelial cells MLE-12 transfected with inhibitors and precursors for miR-30a, miR-30d, miR-23b, miR-125 and miR-337, miR-466a, miR466d, miR-476c, respectively
Organism Mus musculus
Experiment type Expression profiling by array
Summary The regulation of gene expression in cells, including by microRNAs (miRNAs), is a dynamic process. Current methods for identifying microRNA targets by combining sequence, miRNA and mRNA expression data do not adequately utilize the temporal information and thus miss important miRNAs and their targets. We developed a new method, mirDREM, that uses probabilistic modeling to reconstruct dynamic regulatory networks which explain how temporal gene expression is jointly regulated by microRNAs and transcription factors (TFs). We used mirDREM to study the regulation of postnatal lung development in mice. The reconstructed network for this process identified several known miRNAs and TFs and provided novel predictions about additional miRNAs and the specific developmental phases they regulate. Microarray data of Mouse Lung Epithelial cells MLE-12 after transfection with inhibitors for miR-30a, miR-30d, miR-23b and miR-125 and with precursors for miR-337, miR-466a, miR466d and miR-476c. The results provide a general insight into the gene expression profile which was modulated by the inhibition or overexpression of these microRNAs. We experimentally validated several predictions and show that miR-30d, miR-30a, and miR-467c are new regulators of proliferation in lung cells. Our analysis establishes new links between identified miRNAs and lung diseases, supporting recent evidence that such diseases may represent reversal of lung differentiation.
 
Overall design We first analyzed the endogenous expression of miR-30a, miR-30d, miR-23b, miR-125, miR-337, miR-466a, miR466d and miR-476c in MLE-12 cells. Then, we transfected the MLE-12 cells with inhibitors ( miR-30a, miR-30d, miR-23b and miR-125) for the highly expressed and precursos for those that were almost undetectable (miR-337, miR-466a, miR466d and miR-476c). RNA level of the 8 microRNAs was verify by qRT-PCR in order to validate the transfection efficiency. Finally, 0.5ug of total RNA was used to performe the gene expression microarrays for each condition
 
Contributor(s) Cardenas CL, Pandit KV, Kaminski N
Citation(s) 23986498
Submission date Oct 15, 2012
Last update date Feb 02, 2018
Contact name christian lacks lino cardenas
E-mail(s) clinocardenas@mgh.harvard.edu
Phone 4127993484
Organization name Massachusetts general hospital-Harvard medical school
Department division of cardiovascular
Lab Lindsay
Street address 185 Cambridge st Room 3224
City Boston
State/province Massachusetts
ZIP/Postal code 02114
Country USA
 
Platforms (1)
GPL10787 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version)
Samples (40)
GSM1020038 antctrl-1_Mouse Lung Epithelial Cells MLE-12_rep1
GSM1020039 antctrl-2_Mouse Lung Epithelial Cells MLE-12_rep2
GSM1020040 antctrl-3_Mouse Lung Epithelial Cells MLE-12_rep3
Relations
BioProject PRJNA177680

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41607_RAW.tar 126.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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