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Series GSE41616 Query DataSets for GSE41616
Status Public on Jan 08, 2013
Title Identification of Novel Molecular Markers through Transcriptomic Analysis in Human Fetal and Adult Corneal Endothelial Cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Corneal endothelium is composed of a monolayer of corneal endothelial cells (CECs) in the inner layer of cornea, which is essential for maintaining corneal transparency. In order to better characterize CECs in different developmental stages, we profiled mRNA transcriptomes in human fetal and adult corneal endothelium with the goal to identify novel molecular markers in these cells. By comparing CECs with 12 other types of tissues, we identified 245 and 284 signature genes that are highly expressed in fetal and adult CECs, respectively. Functionally, these genes are characteristic of CECs, involving in cell adhesion, proteoglycan and sulfur metabolic process. Importantly, several of these genes are disease target genes in hereditary corneal dystrophies, consistent with their functional significance in CEC physiology. By comparing fetal and adult CECs, we also identified stage-specific markers associated with CEC maturation, such as the activation of the Wnt pathway genes in fetal, but not in adult CECs. Lastly, by immunohistochemistry of ocular tissues, we further confirmed the unique protein expression patterns for Wnt5a, S100A4, S100A6, and IER3, the four novel markers for either fetal or adult CECs. The identification of a new panel of molecular markers for fetal and mature CECs would be very useful for characterizing and quality controlling CECs through ex vivo expansion or stem cell differentiation for cell replacement therapy.
Overall design mRNA profile between adult and fetal CECs by high-throughput sequencing
Contributor(s) Huang K, Fan G
Citation(s) 23257286
Submission date Oct 16, 2012
Last update date May 15, 2019
Contact name Kevin Huang
Phone 310-267-0438
Organization name UCLA
Department Human Genetics
Lab Guoping Fan
Street address Gonda BLDG Rm. 6554, P.O.Box 957088
City Los Angeles
State/province CA
ZIP/Postal code 90095-7088
Country USA
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (5)
GSM1020212 adult CEC 33
GSM1020213 adult CEC 39
GSM1020214 adult CEC 40
BioProject PRJNA177700
SRA SRP016140

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41616_CEC_matrix.txt.gz 334.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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