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Series GSE41696 Query DataSets for GSE41696
Status Public on Oct 19, 2012
Title Temporal expression data from zebrafish pineal gland
Organism Danio rerio
Experiment type Expression profiling by array
Summary Microarray data allowed detection of genes that have circadian expression pattern in the zebrafish pineal gland
Overall design Adult (0.5-1.5 years old) transgenic zebrafish, Tg(aanat2:EGFP)Y8, which express enhanced green fluorescent protein (EGFP) in the pineal gland under the control of the aanat2 regulatory regions, were used. Fish were raised under 12-hr light:12-hr dark (LD) cycles, in a temperature controlled room, and transferred to constant darkness (DD) for tissue collection. Fish were anesthetized in 1.5 mM Tricane (Sigma), sacrificed by decapitation, and pineal glands were removed under a fluorescent dissecting microscope. Starting from circadian time (CT) 14, pineal glands were collected at 4-hr intervals for 48 hours (12 time points identified as CT 14, 18, 22, 2, 6, 10, 14b, 18b, 22b, 2b, 6b and 10b). Pools of 12 pineal glands were prepared at each time-point and total RNA was extracted using the RNeasy Lipid Tissue Mini Kit (QIAGEN), according to the manufacturer's instructions.
Contributor(s) Gothilf Y
Citation(s) 23284293
Submission date Oct 18, 2012
Last update date Aug 23, 2019
Contact name Shahar Alon
Organization name Tel Aviv University
Department Department of Neurobiology, The George S. Wise Faculty of Life Sciences
Lab Yoav Gothilf Lab
Street address Tel Aviv University
City Tel Aviv
ZIP/Postal code 69978
Country Israel
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (12)
GSM1022843 Pineal_DD_CT14
GSM1022844 Pineal_DD_CT18
GSM1022845 Pineal_DD_CT22
BioProject PRJNA177960

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41696_RAW.tar 24.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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