 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 19, 2012 |
| Title |
Temporal expression data from zebrafish pineal gland |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
|
| Summary |
Microarray data allowed detection of genes that have circadian expression pattern in the zebrafish pineal gland
|
| |
|
| Overall design |
Adult (0.5-1.5 years old) transgenic zebrafish, Tg(aanat2:EGFP)Y8, which express enhanced green fluorescent protein (EGFP) in the pineal gland under the control of the aanat2 regulatory regions, were used. Fish were raised under 12-hr light:12-hr dark (LD) cycles, in a temperature controlled room, and transferred to constant darkness (DD) for tissue collection. Fish were anesthetized in 1.5 mM Tricane (Sigma), sacrificed by decapitation, and pineal glands were removed under a fluorescent dissecting microscope. Starting from circadian time (CT) 14, pineal glands were collected at 4-hr intervals for 48 hours (12 time points identified as CT 14, 18, 22, 2, 6, 10, 14b, 18b, 22b, 2b, 6b and 10b). Pools of 12 pineal glands were prepared at each time-point and total RNA was extracted using the RNeasy Lipid Tissue Mini Kit (QIAGEN), according to the manufacturer's instructions.
|
| |
|
| Contributor(s) |
Gothilf Y |
| Citation(s) |
23284293 |
| Submission date |
Oct 18, 2012 |
| Last update date |
Aug 23, 2019 |
| Contact name |
Shahar Alon |
| Organization name |
Tel Aviv University
|
| Department |
Department of Neurobiology, The George S. Wise Faculty of Life Sciences
|
| Lab |
Yoav Gothilf Lab
|
| Street address |
Tel Aviv University
|
| City |
Tel Aviv |
| ZIP/Postal code |
69978 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL1319 |
[Zebrafish] Affymetrix Zebrafish Genome Array |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA177960 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE41696_RAW.tar |
24.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...