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| Status |
Public on Jun 01, 2014 |
| Title |
Transcriptional profiling of human cancer cell lines upon ZMPSTE24 silencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Defining the aging-cancer relationship is a challenging task. Mice deficient in Zmpste24, a metalloproteinase mutated in human progeria and involved in nuclear prelamin A maturation, recapitulate many features of aging. However, their short lifespan and cell-intrinsic and -extrinsic alterations restrict the application and interpretation of carcinogenesis protocols. To circumvent these limitations we have generated Zmpste24 mosaic mice. Interestingly, these mice develop normally - revealing cell-extrinsic mechanisms are preeminent in progeria- and display decreased incidence of infiltrating oral carcinomas. Moreover, ZMPSTE24 knock-down reduces human cancer cell invasiveness. Our results disclose the ZMPSTE24-prelamin A system as an example of antagonistic pleiotropy on cancer and aging, support the potential of cell-based and systemic therapies for progeria, and highlight ZMPSTE24 as a new anticancer target.
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| Overall design |
We used siRNAs to silence ZMPSTE24 in different human cancer cell lines (SCC-40, SCC-2, A549 and MDA-MB-231), and compared their RNA expression profiles with those of mock treated cells. Each experimental condition (ZMPSTE24 or Scrambled siRNA) was performed in duplicate. Thus, a total of 16 array hybridizations were performed.
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| Contributor(s) |
de la Rosa J, Rad R, Bradley A, Cadiñanos J, López-Otín C |
| Citation(s) |
23917225 |
| Submission date |
Oct 23, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Jorge de la Rosa |
| E-mail(s) |
jrs@degradome.uniovi.es
|
| Organization name |
Instituto de Medicina Oncológica y Molecular de Asturias (IMOMA)
|
| Street address |
Avda. Jose Maria Richard
|
| City |
Oviedo |
| ZIP/Postal code |
33193 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
|
| Samples (16)
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| Relations |
| BioProject |
PRJNA178213 |
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