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| Status |
Public on Feb 06, 2013 |
| Title |
Expression profiles of primary bone marrow derived mouse macrophages – untreated and treated with LPS or LPS+PGE |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The polarization of macrophages into an anti-inflammatory or regulatory phenotype plays an important role in resolving inflammation. PGE2 regulates macrophage polarization via a PKA dependent pathway. PKA phosphorylates SIKs, inhibiting their ability to phosphorylate CRTC3 in cells. This in turn allows CRTC3 to translocate to the nucleus where it acts as a co-activator with the transcription factor CREB to induce IL-10 transcription. In line with this we find that either genetic or pharmacological inhibition of SIKs mimics the effect of PGE2 on IL-10 production.
We show here that PGE2, in combination with LPS, is able to promote a regulatory like phenotype in macrophages characterized by high expression of IL-10 as well as the regulatory markers SPHK1 and LIGHT.
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| Overall design |
Gene expression profiles of primary bone marrow derived mouse macrophages treated with LPS or LPS+PGE and untreated controls were generated using Affymetrix mouse gene 1.1 ST arrays. In total, 12 arrays were generated from the three groups (LPS, LPS+PGE and control) with 4-replicates in each group. One sample from LPS group was removed due to QC issues and the remaining 11 that were used in our analyses are submitted here.
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| Contributor(s) |
Arthur SJ, Mudaliar MA, Barton GJ, Elcombe S, MacKenzie K |
| Citation(s) |
23241891 |
| Submission date |
Oct 25, 2012 |
| Last update date |
Apr 18, 2017 |
| Contact name |
Manikhandan A V Mudaliar |
| E-mail(s) |
innovatebio@gmail.com
|
| Organization name |
University of Glasgow
|
| Department |
College of Medical, Veterinary and Life Sciences
|
| Lab |
Institute of Infection, Immunity and Inflammation
|
| Street address |
Room B528, Sir Graeme Davies Building, 120 University Place
|
| City |
Glasgow |
| State/province |
Scotland |
| ZIP/Postal code |
G12 8TA |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (11)
|
| GSM1025301 |
macrophages_untreated_control_rep4 |
| GSM1025302 |
macrophages_treated_with_LPS_1hr_rep_1 |
| GSM1025303 |
macrophages_treated_with_LPS_1hr_rep_2 |
| GSM1025304 |
macrophages_treated_with_LPS_1hr_rep_3 |
| GSM1025305 |
macrophages_treated_with_LPS+PGE_1hr_rep_1 |
| GSM1025306 |
macrophages_treated_with_LPS+PGE_1hr_rep_2 |
| GSM1025307 |
macrophages_treated_with_LPS+PGE_1hr_rep_3 |
| GSM1025308 |
macrophages_treated_with_LPS+PGE_1hr_rep_4 |
|
| Relations |
| BioProject |
PRJNA178264 |
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