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Status |
Public on Oct 26, 2012 |
Title |
Expression data from control and LRF (leukemia/lymphoma related factor)-deficient mouse LT-HSCs |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
LRF, which is encoded by the ZBTB7A gene and formerly known as POKEMON (POK erythroid myeloid ontogenic factor), was originally identified as a PLZF (promyelocytic leukemia zinc finger) homologue interacting with BCL6 (B-cell lymphoma 6). LRF is a transcription factor that is broadly expressed in hematopoietic lineage cells, but its expression is particularly high in erythroblasts and germinal center (GC) B-cells. The goal of this study is to assess the effect of LRF loss on the LT-HSC transcriptome. Nine days after injection of adult mice with polyinosinic polycytidylic acid (pIpc) to activate Cre, total RNAs were isolated from double-sorted LT-HSCs from LRF Flox/+ Mx1-Cre+ and LRF Flox/Flox Mx1-Cre+ mice and processed for microarray analysis.
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Overall design |
We performed gene expression microarray analysis of FACS-sorted LT-HSCs (LSK IL7Ra-Flt3-CD150+CD48-) to assess the effect of Lrf loss on the LT-HSC transcriptome. Zbtb7a Flox/+ Mx1-Cre+ mice were used as a control to normalize the potential effects of Cre recombinase. LT-HSCs were FACS-sorted from three Lrf knockout (Zbtb7a Flox/Flox Mx1-Cre+) and two control (Zbtb7a Flox/+ Mx1-Cre+) mice, nine days after the first pIpC injection.
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Contributor(s) |
Lee SU, Li SM, Maeda T |
Citation(s) |
23134786 |
Submission date |
Oct 25, 2012 |
Last update date |
Feb 11, 2019 |
Contact name |
Takahiro Maeda |
E-mail(s) |
tmaeda@partners.org
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Organization name |
Brigham and Women's Hospital
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Street address |
One Blackfan Circle
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
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Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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Samples (5)
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Relations |
BioProject |
PRJNA178296 |