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Series GSE4189 Query DataSets for GSE4189
Status Public on Feb 08, 2006
Title The Oct4 and Nanog transcription network that regulates pluripotency in mouse embryonic stem cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary A) We profiled gene expression changes during ES cell differentiation

B) We profiled gene expression changes when Pou5f1 or Nanog is knockdown upon RNAi
Keywords: Array Based
 
Overall design A) Mouse ES cells were induced to differentiate by 3 different chemical treatments. The RNAs were hybridized onto custom-made microarrays.

B) We used siRNA to knockdown Pou5f1 or Nanog in mouse ES cells. The RNAs from control and knockdown cells were hybridized onto Affymetrix Mouse Genome 430 2.0 microarrays
 
Contributor(s) Loh Y, Wu Q, Chew J, Vega VB, Chang W, Chen X, Bourque G, George J, Leong B, Liu J, Wong K, Sung KW, Lee CH, Zhao X, Chiu K, Lipovich L, Kuznetsov VA, Robson P, Stanton LW, Wei C, Ruan Y, Lim B, Ng H
Citation(s) 16518401
Submission date Feb 07, 2006
Last update date Feb 11, 2019
Contact name Huck Hui Ng
E-mail(s) nghh@gis.a-star.edu.sg
Phone +65 64788145
Fax +65 64789004
URL http://www.gis.a-star.edu.sg
Organization name Genome Institute of Singapore
Department Cell and Medical Biology
Lab Cell and Medical Biology
Street address 60 Biopolis street, #02-01 Genome
City Singapore
State/province Singapore
ZIP/Postal code 138672
Country Singapore
 
Platforms (2)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
GPL3440 GIS 22k Compugen Mm Oligo Microarray
Samples (32)
GSM94856 Parental vector, pSUPER-puro replicate 1
GSM94857 Parental vector, pSUPER-puro replicate 2
GSM94858 Parental vector, pSUPER-puro replicate 3
Relations
BioProject PRJNA95037

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4189_RAW.tar 118.9 Mb (http)(custom) TAR (of CEL, GPR)
Raw data provided as supplementary file

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