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Series GSE41892 Query DataSets for GSE41892
Status Public on Oct 29, 2012
Title Tetraspanin TSPAN12 regulates tumor growth and metastasis and inhibits β-catenin degradation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Ablation of tetraspanin protein TSPAN12 from human MDA-MB-231 cells significantly decreased primary tumor xenograft growth, while increasing tumor apoptosis. Furthermore, TSPAN12 removal markedly enhanced tumor-endothelial interactions and increased metastasis to mouse lungs. TSPAN12 removal from human MDA-MB-231 cells also caused diminished association between FZD4 (a key canonical Wnt pathway receptor) and its co-receptor LRP5. The result likely explains substantially enhanced proteosomal degradation of β-catenin, a key effecter of canonical Wnt signalling. Consistent with disrupted canonical Wnt signaling, TSPAN12 ablation altered expression of LRP5, Naked 1 and 2, DVL2, DVL3, Axin 1 and GSKβ3 proteins. TSPAN12 ablation also altered expression of several genes regulated by β-catenin (e.g. CCNA1, CCNE2, WISP1, ID4, SFN, ME1) that may help to explain altered tumor growth and metastasis. In conclusion, these results provide the first evidence for TSPAN12 playing a role in supporting primary tumor growth and suppressing metastasis. TSPAN12 appears to function by stabilizing FZD4-LRP5 association, in support of canonical Wnt-pathway signaling, leading to enhanced β-catenin expression and function.
 
Overall design 4 samples = 2 Control + 2 TSPAN12KD
 
Contributor(s) Knoblich K, Sharma C, Wang H, Fletcher AL, Turley SJ, Hemler ME
Citation(s) 23955570
Submission date Oct 28, 2012
Last update date Dec 06, 2018
Contact name Konstantin Knoblich
Organization name Monash University
Department Biochemistry and Molecular Biology
Lab STIMMLAB
Street address 15 Innovation Walk
City Clayton
State/province VIC
ZIP/Postal code 3800
Country Australia
 
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (4)
GSM1027065 MFP tumor from Control cells 1
GSM1027066 MFP tumor from Control cells 2
GSM1027067 MFP tumor from TSPAN12 KD cells 1
Relations
BioProject PRJNA178411

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41892_RAW.tar 8.1 Mb (http)(custom) TAR (of CEL)
GSE41892_TSPAN12KD.gct.txt.gz 1.2 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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