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| Status |
Public on Oct 30, 2012 |
| Title |
A global transcriptome analysis of keratinocytes upon suppression of endogenous microRNA-31 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
MiR-31 is one of the most highly overexpressed miRNAs in psoriasis skin; however, its biological role in the disease has not been studied. Here we show that miR-31 is markedly overexpressed in psoriasis keratinocytes. To study the biological role of miR-31 in keratinocytes, we transfected miR-31 hairpin inhibitor (anti-miR-31) into primary human keratinocytes to inhibit endogenous miR-31. We performed a global transcriptome analysis of keratinocytes upon suppression of endogenous miR-31 using Affymetrix arrays.
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| Overall design |
Expression profiling of primary human keratinocytes transfected with 10nM miR-31 hairpin inhibitor (anti-miR-31) or control hairpin RNA (anti-miR-Ctrl) for 48 hours (biological triplicates in each group) was performed using the Affymetrix GeneTitan system.
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| Contributor(s) |
Xu N, Meisgen F, Butler LM, Han G, Wang XJ, Söderberg-Nauclér C, Ståhle M, Pivarcsi A, Sonkoly E |
| Citation(s) |
23233723 |
| Submission date |
Oct 29, 2012 |
| Last update date |
Nov 08, 2016 |
| Contact name |
Ning Xu |
| Organization name |
Karolinska Institutet
|
| Street address |
CMM L8:02
|
| City |
Stockholm |
| ZIP/Postal code |
17176 |
| Country |
Sweden |
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| Platforms (1) |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA178462 |
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