|
| Status |
Public on Oct 03, 2013 |
| Title |
Global analysis of off-target genes by GAPDH siRNA |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
A GAPDH siRNA was overexpressed in HeLa cells and microarray expression profiling was performed to experimentally identify the off-targets of this siRNA at the transcriptome level. Among all predicted siRNA off-targets by MirTarget2, the majority were downregulated by at least 10% (i.e. less than 90% remaining gene expression). Moreover, more than one fifth were downregulated by at least 30%. The gene knockdown data is consistent with previous observations that siRNA off-targets are generally silenced at a more moderate level as compared to silencing of the single intended target
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| |
|
| Overall design |
GAPDH siRNA was transfected into HeLa cells, and the unintended off-targets was identified by microarrays 24 hours post transfection.
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| |
|
| Contributor(s) |
Wang X |
| Citation(s) |
24145823 |
| Submission date |
Oct 31, 2012 |
| Last update date |
Aug 16, 2018 |
| Contact name |
Xiaowei Wang |
| Organization name |
University of Illinois at Chicago
|
| Street address |
909 S Wolcott Ave, Rm 5137
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60612 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6947 |
Illumina HumanHT-12 V3.0 expression beadchip |
|
| Samples (3) |
|
| Relations |
| BioProject |
PRJNA178530 |
