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Series GSE4193 Query DataSets for GSE4193
Status Public on Feb 09, 2006
Title Gene expression in murine germ cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary In mammals, the X and Y chromosomes are subject to meiotic sex chromosome inactivation (MSCI) during prophase I in the male germline, but their status thereafter is currently unclear. An abundance of X-linked spermatogenesis genes has spawned the view that the X must be active [1-8]. On the other hand, the idea that the imprinted paternal X of the early embryo may be pre-inactivated by MSCI suggests that silencing may persist longer [9-12]. To clarify this issue, we establish a comprehensive X-expression profile during mouse spermatogenesis. Here, we discover that the X and Y occupy a novel compartment in the post-meiotic spermatid and adopt a non-Rabl configuration. We demonstrate that this post-meiotic sex chromatin (PMSC) persists throughout spermiogenesis into mature sperm and exhibits epigenetic similarity to the XY body. In the spermatid, 87% of X-linked genes remain suppressed post-meiotically, while autosomes are largely active. We conclude that chromosome-wide X-silencing continues from meiosis to the end of spermiogenesis and discuss implications for proposed mechanisms of imprinted X-inactivation.
Keywords: Specific germ cells from murine testis
 
Overall design Independent germ cell preps were used for array analysis. Duplicates were provided for each sample.
 
Citation(s) 16581510
Submission date Feb 07, 2006
Last update date Feb 11, 2019
Contact name Lizhong Yang
E-mail(s) lzyang@wsu.edu
Phone 1-509-335-2240
Fax 1-509-335-9688
URL http://www.wsu.edu/~griswold
Organization name Washington State University
Department School of Molecular Biosciences
Lab Griswold Lab/Center for Reproductive Biology
Street address 531 Fulmer Hall/POB 644660
City Pullman
State/province WA
ZIP/Postal code 99164-4660
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM95928 Type A spermatogonia replicate 1
GSM95929 Type A spermatogonia replicate 2
GSM95930 Type B spermatogonia replicate 1
Relations
BioProject PRJNA95043

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Supplementary file Size Download File type/resource
GSE4193_RAW.tar 53.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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