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Series GSE42139 Query DataSets for GSE42139
Status Public on Apr 15, 2013
Title Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules
Organism Mus musculus
Experiment type Expression profiling by array
Summary Anterior foregut endoderm (AFE) gives rise to many tissue types of interest for therapeutic research including the esophagus, salivary glands, lung, thymus, parathyroid and thyroid. Despite its importance, only few reports describe the generation of AFE from pluripotent stem cells (PSCs) by directed differentiation. Here, we describe a novel protocol to derive a subdomain of AFE, identified by expression of Pax9, from PSCs using small molecules and chemically defined conditions. Generation of a reporter PSC line allows isolation and characterization of Pax9+ AFE cells. When transplanted in vivo, Pax9+ AFE can form several distinct types of complex anterior foregut epithelia including mucosal glands and stratified squamous epithelium. Finally, we show that the directed differentiation protocol can be used to generate AFE from DiGeorge Syndrome patient-specific human induced PSCs, thus creating a platform to produce anterior foregut derivatives for therapy and to enable the study of disorders of the AFE.
Overall design Total RNA obtained from FACS purified from in vitro dervied mouse definitive endoderm, anterior foregut and ES cells. AFE cells were derived from a 129X1/SvJ background, DE cells from 129X1/SvJ x 129S1/SV-+p+Tyr- cKitlSl-J/+ (R1 ES cells) and non reporter ES cells from a 129P2/OlaHsd background.
Contributor(s) Maehr R
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Submission date Nov 08, 2012
Last update date Jun 14, 2018
Contact name Michael Johannes Ziller
Organization name Max Planck Institute of Psychiatry
Department Translational Psychiatry
Lab Ziller lab
Street address Kraepelinstrasse 2-10
City Munich
ZIP/Postal code 80804
Country Germany
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (9)
GSM1033415 in vitro derived definitive endoderm 1
GSM1033416 in vitro derived definitive endoderm 2
GSM1033417 in vitro derived definitive endoderm 3
BioProject PRJNA179199

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42139_RAW.tar 3.1 Mb (http)(custom) TAR
GSE42139_non-normalized_data.txt.gz 1.5 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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