|
| Status |
Public on Dec 07, 2012 |
| Title |
RECURRENT SETBP1 MUTATIONS IN ATYPICAL CHRONIC MYELOID LEUKEMIA |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-Seq analysis of atypical chronic myeloid leukemia samples
|
| |
|
| Overall design |
We sequenced leukemic mRNA from 13 Atypical Cronic Mieloid Leukemia (aCML) samples by Illumina GAIIx. Transcriptomic profiles, differentially expressed genes and pathway enrichment analysis were obtained comparing 7 SETBP1-mutated samples and 6 non-mutated (WT) samples by using TopHat aligner and SAMMate gene expression quantifier. We focused on the gene expression profile of known coding transcripts. A dataset of 20,907 protein-coding Ensembl Genes was obtained from the RNA-Seq by using the Human Ensembl GTF annotation file vs54 dowloaded from ftp://ftp.ensembl.org/pub/release-54/gtf/homo_sapiens/.
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| |
|
| Contributor(s) |
Spinelli R, Piazza R, Pirola A, Gambacorti-Passerini C |
| Citation(s) |
23222956 |
| Submission date |
Nov 08, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Roberta Spinelli |
| E-mail(s) |
roberta.spinelli@unimib.it
|
| Phone |
0039-02-64488032
|
| Fax |
0039-02-6448-8260
|
| URL |
http://www.dimep.medicina.unimib.it/personale_175.php?docente_id=32
|
| Organization name |
University of Milano Bicocca
|
| Department |
Department of Health Science
|
| Lab |
Molecular Oncology Laboratory
|
| Street address |
Via Cadore 48
|
| City |
Monza |
| State/province |
MB |
| ZIP/Postal code |
20900 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (13)
|
|
| Relations |
| BioProject |
PRJNA179284 |
| SRA |
SRP017138 |
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