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| Status |
Public on Feb 06, 2013 |
| Title |
Probiotic and Prebiotic Supplementation of Antiretroviral Therapy Improves Gastrointestinal Immunity in SIV-infected Macaques |
| Platform organism |
Homo sapiens |
| Sample organism |
Macaca nemestrina |
| Experiment type |
Expression profiling by array
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| Summary |
HIV infection results in damage to the gastrointestinal (GI) tract, microbial translocation and immune activation, which are not completely ameliorated with suppression of viremia by antiretroviral (ARV) therapy. Furthermore, increased morbidity and mortality of ARV-treated HIV-infected individuals is associated with these dysfunctions. Thus, in order to enhance GI tract immunity, we treated SIV-infected pigtail macaques with ARVs supplemented with probiotics and prebiotics or with ARVs alone. In the colon, this synbiotic treatment resulted in increased expression of genes associated with antigen presenting cells (APCs), increased frequency and functionality of APCs, enhanced reconstitution and functionality of CD4+ T-cells and reduced fibrosis of lymphoid follicles. Thus, supplementing ARV treatment with synbiotic treatment in HIV-infected individuals may improve GI tract immunity and thereby mitigate inflammatory sequelae, ultimately improving prognosis.
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| Overall design |
CD45+ (clone MB4-6D6) leukocytes were sorted from thawed colon samples from 4 ARV + probiotics animals and 4 ARV alone animals
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| Contributor(s) |
Canary LA, Sun X, Vinton CL, Funderburg NT, Morcock DR, Quiñones M, Deming CB, Perkins M, Hazuda DJ, Miller MD, Lederman MM, Segre JA, Lifson JD, Haddad EK, Estes JD, Brenchley JM, Wilkinson PA |
| Citation(s) |
23321668 |
| Submission date |
Nov 13, 2012 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Peter A Wilkinson |
| Organization name |
Case Western Reserve University
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| Department |
Pathology / Systems Biology & Bioinformatics
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| Street address |
2103 Cornell Road
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| City |
Cleveland |
| State/province |
Ohio |
| ZIP/Postal code |
44120 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA179375 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42232_RAW.tar |
28.3 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE42232_non-normalized_data.txt.gz |
659.3 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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