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Series GSE42277 Query DataSets for GSE42277
Status Public on Dec 10, 2012
Title Transcriptional Reprogramming of Mature CD4 T helper Cells generates distinct MHC class II restricted Cytotoxic T Lymphocytes
Organism Mus musculus
Experiment type Expression profiling by array
Summary We show here that the T helper-fate is not fixed and that mature antigen-stimulated CD4 T cells can switch off Thpok expression and reactivate CD8- lineage genes. This unexpected plasticity results in the post-thymic termination of the T helper- program and the functional differentiation of distinct MHC class II restricted CD4 cytotoxic T lymphocytes
Overall design Small intestine CD4 intraepithelial T lymphocytes from ThPOK-GFP reposter mice were isolated and sorted (FACS Aria) based on ThPOK and CD8aa expression. Cell were isolated either from non-experimental ThPOK-GFP reporter mice (WT) or after transfering CD4 naive T cells from ThPOK-GFP reporter mice to RAG-/-recipient animals (TR) as an experimental colitis model. Experimet was done in duplicate.
Contributor(s) Mucida D, Reis BS
Citation(s) 23334788
Submission date Nov 14, 2012
Last update date Jun 14, 2018
Contact name Bernardo Reis
Organization name Rockefeller University
Street address 1234 York Avenue
City New York
State/province NY
ZIP/Postal code 10065
Country USA
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (12)
GSM1036847 THPOK+CD8a-_1_WT
GSM1036848 THPOK+CD8a-_2_WT
GSM1036849 THPOK-CD8a-_1_WT
BioProject PRJNA179517

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42277_RAW.tar 3.1 Mb (http)(custom) TAR
GSE42277_non-normalized.txt.gz 3.7 Mb (ftp)(http) TXT
Raw data are available on Series record
Processed data included within Sample table

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