|
| Status |
Public on Jul 05, 2013 |
| Title |
Integrative genomics of gene regulation by estrogen receptors and and coregulators [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The closely related transcription factors (TFs), estrogen receptors ERα and ERβ, regulate divergent gene expression programs and proliferative outcomes in breast cancer. Utilizing MCF-7 breast cancer cells with ERα, ERβ, or both receptors as a model system to define the basis of differing response specification by related TFs, we show that these TFs and their key coregulators, SRC3 and RIP140, generate overlapping as well as unique chromatin-binding and transcription-regulating modules. Cistrome and transcriptome analyses and use of clustering algorithms delineated 11 clusters representing different chromatin-bound receptor and coregulator assemblies that could be functionally associated through enrichment analysis with distinct patterns of gene regulation and preferential coregulator usage, RIP140 with ERβ and SRC3 with ERα. The receptors modified each other’s transcriptional effect, and ERβ countered the proliferative drive of ERα through several novel mechanisms associated with specific binding site clusters. Our findings delineate distinct TF-coregulator assemblies that function as control nodes specifying precise patterns of gene regulation, proliferation, and metabolism, as exemplified by two of the most important nuclear hormone receptors in human breast cancer.
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| |
|
| Overall design |
Examination of Estrogen Receptors alpha and beta and their coregulators SRC3 and RIP140 in different cell types
|
| |
|
| Contributor(s) |
Erdogan ZM, Katzenellenbogen BS |
| Citation(s) |
23774759 |
| Submission date |
Nov 16, 2012 |
| Last update date |
Feb 21, 2019 |
| Contact name |
Zeynep Madak Erdogan |
| Organization name |
UIUC
|
| Department |
FSHN
|
| Lab |
Zeynep Madak Erdogan
|
| Street address |
1201 S Goodwin Ave
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
|
| Samples (10)
|
|
| This SubSeries is part of SuperSeries: |
| GSE42349 |
Integrative genomics of gene regulation by estrogen receptors and and coregulators |
|
| Relations |
| BioProject |
PRJNA181136 |
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